慢性乙型肝炎和肝硬化中乙型肝炎病毒X抗原表达的分析  

ANALYSIS OF HEPATITIS B VIRUS XANTIGEN EXPRESSION IN CHRONIC HEPATITIS B AND CIRRHOSIS

作　　者：黄耀煊[1] 邬光惠[1] 王锡育[1] 吴霞[1] 范公忍[1] 邓燕林[1] 皋岚湘[1] 黄洁[1] 

机构地区：[1]北京军区总医院肝病研究所,白求恩国际和平医院传染科,北京军区总医院病理科

出　　处：《中华内科杂志》1995年第4期235-238,共4页Chinese Journal of Internal Medicine

摘　　要：用基因工程技术由大肠杆菌合成重组乙型肝炎病毒（ＨＢＶ）Ｘ抗原（ＨＢｘＡｇ）并制备兔抗－ＨＢｘＩｇＧ以检测ＨＢｘＡｇ，合成一对以ＨＢＶＸ基因序列为模板的引物，用聚合酶链反应（ＰＣＲ）技术扩增ＨＢＶＤＮＡ。免疫组织化学和血清学方法分别用以分析肝病患者肝组织中的ＨＢｘＡｇ和血清标本中的ＨＢｘＡｇ、抗－ＨＢｘ。发现ＨＢｘＡｇ在慢性活动性肝炎（ＣＡＨ）患者肝组织中检出率为７２．７％，在肝炎后肝硬化（ＬＣ）中为９２．６％，而乙型肝炎核心抗原（ＨＢｃＡｇ）在ＬＣ中检出率为４７．８％。在ＣＡＨ、慢性迁延性肝炎和ＬＣ的血清中ＨＢｘＡｇ的阳性率分别为４４．４％、６６．６％和３３．３％，与ＨＢｅＡｇ的阳性率相似，而且在这些ＨＢｘＡｇ阳性的血清中可检出ＨＢＶＤＮＡ的存在，ＨＢｘＡｇ的表达与ＨＢＶ复制紧密相关，ＨＢｘＡｇ可能是一慢性ＨＢＶ感染的重要标志物。t has been shown that hepatitis B virus(HBV)X antigen(HBxAg)functioned as a transactivatingelement which can act on the enhancer of HBV in an in vitro svstem and elevate the transcriptional level ofHBV. In this study we investigate the relationship between HBxAg expression and HBV replication in pa-tients with chronic hepatitis B and clrrhosis. Rabbit IgG against recombinant HBxAg which was synthe-sized in E,coli were prepared and used for the detection of HBxAg. HBV was DNA was amplified by poly-merase chain reaction technique by using primers from HBx gene sequence. Liver tissue samples and serafrom the patients were examined immunohistochemically for HBxAg and serologically for HBxAg/anti-HBx respectively. We focused on its expression in these samples in comparison with markers of HBV repli-cation. It was found that in liver HBxAg was present in 72.7% of the patients with chronic active hapatitis(CAH)and 92.6% of those with cirrhosis,while the positivity rate of HBcAg in cirrhosis patients was on- ly 47.8%. In the sera of the patients with CAH, chronic persistant hapatitis and cirrhosis HBxAg waspresent in 44.4%,66.6%and 33.3% respectively. It was similar to that observed with HBeAg.More-over in these HBxAg positive sera HBV DNA can also be detected.It was shown that higher rale of posi-tivity of HBxAg was found in patients with replicative markers (serum HBeAg,serum HBV DNA or livertissue HBcAg positive). Our results indicate that expression of HBxAg is closelv correlated with HBVreplication and HBxAg may be an important marker in chronic HBV infection.

关 键 词：乙型肝炎 肝硬变 乙型肝炎病毒 X抗原 

分 类 号：R512.603[医药卫生—内科学]