单结合胆红素促成核活性的研究  

An observation on nucleating activity of monoconjugat-ed bilirubin(MCB ) in model bile system

作　　者：王人颢[1] 祝学光[1] 刘玉京 韩建德[1] 

机构地区：[1]北京医科大学人民医院外科

出　　处：《中华外科杂志》1995年第11期662-665,共4页Chinese Journal of Surgery

基　　金：国家自然科学基金

摘　　要：作者目的在于探讨人类胆汁中存在的一种具有沉淀特性的胆红素组份，单结合胆红素（ＭＣＢ）对胆固醇过饱和胆汁可能具有的促成核活性。作者应用本实验室已建立的胆红素高效液相色谱法分析技术，从人胆汁中首先获得结合型胆红素，再经分离、纯化及二次制备手段提取ＭＣＢ。然后按照Ｋｉｂｅ氏配方，制备出胆固醇饱和指数为１．２，总脂浓度为１０ｇ／ｄｌ，ＢＳ／ＰＬ为２．３的人工模拟胆汁，并观察在该胆汁中分别加入ＭＣＢ１７０μＭ、ＭＣＢ３４０μＭ时成核时间（ＮＴ）的变化，并与对照组对比，结果显示：（１）三组ＮＴ值依次为３．３３±０．５２天，２．１７±０．４１天和５．８３±０．７５天；（２）无论是两实验组分别与对照组相比或两实验组间ＮＴ值比较，均Ｐ＜０．０５；（３）按照Ｈｏｌｚｂａｃｈ计算两组与对照组ＮＴ值得出成核活性分别为０．５７１２和０．３７２２。证实ＭＣＢ在过饱和的人工模拟胆汁中具有明显的与剂量有关的促成核活性（ＮＡ）。当其剂量达到３４０μＭ时，其成核活性甚至比糖蛋白（ＮＡ为０．５６３６）更强。AbstractRecently, the increasing data suggest that thepathogenesis of cholesterol gallstones may be related tothe imbalance between pro- and anti-nucleating factorsin bile. Our previous results have shown that mono-conjugated bilirubin (MCB)possessed certain pro-nu-cleating activity and might play an important role in theformation of gallstones. The present study was made toobserve the effect of MCB on nucleating activity by us-ing our previous established methods of purificationand preparation of MCB in model system of supersatu-rated bile according to Kibe′s method with cholesterolsaturation index of 1.2, total lipid concentration of10g/dl,and bile salt phospholipid ratio of 2.3. Nucle-ation time(NT) was observed in 3 experimentalgroups with six sarnples each and 2 different contentsof lyophilized MCB was added to above model bile ren-dered a final MCB concentration of(1) 170μM;(MCB-1group)(2)340μM;(MCB-2 group) and(3) no MCBin the as control group.The results were NT 3.33 ±0.52,2.17±0.41 and 5.83±0.75 days in groups.1,2and 3 respectively with the P value<0. 05 when com-pared between the 2 groups. The nucleating activity(NA)value was 0.5712 and 0.3722 in groups 1 and 2,which revealed that the NA elevated in parallele withthe dose of MCB added in model bile,Our findings sug-gust that MCB has a strong pro-nucleating activity inmodel supersaturated biles,and MCB may participatein the formation of cholesterol gallstones throngh itsspecial pro-nucleating activity.

关 键 词：胆固醇 胆结石 胆汁 促成核因子 胆红素 

分 类 号：R657.420.2[医药卫生—外科学] R575.620.2[医药卫生—临床医学]