几种皮肤病皮损处表皮角蛋白组分的研究  

Immunohistological Study of Different Keratins on Lesional Epidermis in Some Dermatoses

作　　者：白兆震[1] 何春涤[1] 王雅坤[1] 袁景涛[1] 王凤贤[1] 陈洪铎[1] 

机构地区：[1]中国医科大学第一临床学院皮肤科,沈阳110001

出　　处：《中华皮肤科杂志》1995年第4期216-218,共3页Chinese Journal of Dermatology

摘　　要：应用一组抗角蛋白单克隆抗体,对慢性湿疹、寻常型银屑病、扁平苔藓、汗孔角化症、脂溢性角化症、基底细胞癌(基癌)及鳞状细胞癌(鳞癌)皮损共37份冰冻标本进行了ABC免疫组化研究。结果表明:这些皮肤病皮损处表皮AE1和AE2阳性角朊细胞分布异常,而AE3、AE21和AE22阳性角朊细胞与在正常的表皮分布相似;扁平苔藓的基底层细胞与AE14反应明显减弱,甚至消失;基癌与鳞癌的瘤细胞均与AE1反应;AE14仅与基癌癌巢周边的细胞反应而不与鳞癌的癌细胞反应。本研究可能有助于深入认识某些皮肤病的发病机理,并为其诊断、鉴别诊断提供有价值的线索。Employing ABC immunoperoxidase technique, thirty-seven frozen sections of biopsy specimens from chronic eczema, psoriasis vulgaris, lichen planus, porokeratosis, seborrheic keratoses, basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) were examined with a panel of anti-keratin monoclonal antibodies (AE1, AE2, AE3, AE14, AE21 and AE22). The results indicated that: ① There was an abnormal reaction of AE1 and AE2 positive keratinocytes (KCs) in epidermal lesions of these dermatoses, but the reactive patterns with AE3, AE21 and AE22 on lesional KCs were similar to those of normal skin; ② The reactivity with AE14 on the basal cells in lichen planus weakened markedly, or even diminished; ③ The tumor cells of both BCC and SCC reacted with AE1, but AE14 reacted only with the tumor ceils on the periphery of the BCC nests, but did not react with those in SCC nests. These might be helpful for the further understanding of the pathogenesis of some skin diseases, and may be of significance for the diagnosis and differential diagnosis.

关 键 词：皮肤病 角蛋白 单克隆抗体 免疫组织化学 

分 类 号：R751.02[医药卫生—皮肤病学与性病学] R751.04[医药卫生—临床医学]