Langerhans细胞组织细胞增生症的免疫组化电镜及DNA流式细胞计数研究  

Immunohistochemical, Ultrastructural Studies and Flow Cytometric DNA Analysis in Langerhans Cell Histiocytosis

作　　者：王健美[1] 唐曙[1] 陈仁贵[1] 凌波[1] 

机构地区：[1]上海第二医科大学附属新华医院皮肤科

出　　处：《中华皮肤科杂志》1995年第4期222-225,共4页Chinese Journal of Dermatology

摘　　要：免疫组化、电镜及流式细胞计数对15例Langerhans细胞组织细胞增生症的皮肤活检标本进行了研究。15例中13例免疫组化染色对抗S100蛋白、花生凝集素反应阳性,对抗Mac387和溶菌酶反应阴性;3例CD_1免疫组化染色均呈阳性;3例电镜观察1例示Birbeck颗粒;10例DNA含量流式细胞仪测定6例为异倍体,DNA指数为1.38±0.32,增殖指数为38.69%。检测结果表明:本病皮损中病理组织细胞来源于Langerhans细胞,且本病存在潜在恶变的可能。The skin biopsy specimens from fifteen patients with Langerhans cell histiocytosis were studied. Thirteen out of fifteen cases were positive to S100 protein antibody and peanut agglutinin, but negative to Mac387 and lysozyme antibodies, and three cases were positive for CD1 antibody by immunohistochemistry. Birbeck granules were demonstrated in one of the three cases by electron microscopy. Ten cases were analysed by flow cytometry, six cases (60%) were aneuploid, DNA index was 1.38±0.32, proliferative index was 38.69%. These results showed that these pathognomonic histiocytes were of Langerhans cell origin and their potential malignancy could not be ruled out.

关 键 词：组织细胞增多症 免疫组织化学 电子显微镜 DNA 

分 类 号：R758.404[医药卫生—皮肤病学与性病学]