Percoll密度梯度离心技术分离纯化豚鼠表皮Langerhans细胞　  

Isolaton and Purification of Epidermal Langerhans Cells from Guinea--pig with Percoll Density Gradient Centrifugation Technique

作　　者：郑茂荣[1] 范青源[1] 谢勇[1] 牟贤龙[1] 方跃明[1] 顾美芳[1,2] 

机构地区：[1]第二军医大学长海医院皮肤科,上海200433 [2]密度梯度离心

出　　处：《中华皮肤科杂志》1995年第5期296-298,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助课题

摘　　要：表皮Ｌａｎｇｅｒｈａｎｓ细胞（ＬＣ）仅占表皮细胞的２％～４％，但在免疫系统中占重要作用。为了对ＬＣ的表型、功能特点及分子生物学水平等作进一步研究，有必要获得高纯化率且具有活性的ＬＣ。我们采用连续、间断Ｐｅｒｃｏｌｌ密度梯度离心相结合的方法分离、纯化豚鼠表皮ＬＣ。结果表明ＬＣ纯化率超过６０％，产率超过８０％，细胞活性超过９０％。自体混合白细胞应答检测证实纯化的ＬＣ仍保持功能活性。该方法简单、快速、价廉，且获得非表面标记性的ＬＣ。Although epidermal Langerhans cells (LC) account for only 2%~4% of epidermal cells,they play an important role in the immune system. In order to investigate the phenotype,functional feature, and molecular biology of LC,it is necessary to obtain the LCs with high purity and activity. A combination of continuous and interval Percoll density gradient centrifugation technique was used to isolate and purify epidermal LC from guinea-pig. The results showed that the LC purity rate was more than 60%,yield rate more than 80 %,and cell activity more than 90 %. Auto--mixed leucocyte response assay showed that the functional activity of LC remained. It is the author's opinion that this method is not only simple, quick,and cheap,but can obtain LC with no surface marker.

关 键 词：LANGERHANS细胞 密度梯度离心 表皮 豚鼠 

分 类 号：R329－24[医药卫生—人体解剖和组织胚胎学] R392.12[医药卫生—基础医学]