原位杂交法检测柯萨奇B3病毒感染鼠各脏器内病毒核酸的动态分布  

In Situ Hybridization of Coxsackie Virus B3 Genome Distribution in Organs of Infected Mice

作　　者：杜晓娟[1] 孙国贤[1] 陈虹[1] 张志 

机构地区：[1]首都儿科研究所,中国军事医学科学院六所

出　　处：《中华实验和临床病毒学杂志》1995年第1期50-52,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：用柯萨奇Ｂ３病毒（ＣｏｘｓａｃｋｉｅｖｖｉｒｕｓＢ３，ＣＶＢ３）ｃＤＮＡ探针原位杂交法检测感染后１２ｄ内小鼠各脏器内病毒核酸的分布。于不同时期分别在心肌细胞、骨骼肌细胞、胰腺细胞、胸腺细胞、脾脏红髓细胞、直肠上皮细胞和肾小管上皮细胞中检测到病毒核酸。为ＣＶＢ３对多脏器的侵犯提供了直接的依据。We have developed a method of in situ hybridization to study the distribution of Coxsackie virus B3(CVB3) genome in the frozen organ sections of mice infected with CVB3 at different intervals in a period of 12 days postinfection. CVB3 genomes were found in myocardial cells, skeletal muscle cells、pancreas cells、lymphocytes of thymus and spleen and epithelial cells of rectum and renal tubules.The results demonstrated that CVB3 might damage multiple organs of infected mice.

关 键 词：原位杂交 DNA探针 柯萨奇病毒 感染 病毒 核酸 

分 类 号：R512.502[医药卫生—内科学]