猪型流感病毒血凝素基因的核苷酸全序列分析  

Analysis of Nucleotide Sequence of Haemagglutinin Gene of Swine Influenza A Virus

作　　者：郭元吉[1] 王敏[1] RGWebster 

机构地区：[1]北京中国预防医学科学院病毒学研究所,美国St.Jude儿童研究医院

出　　处：《中华实验和临床病毒学杂志》1995年第1期11-14,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：对我国大陆首次从猪群中分离到的猪型（Ｈ１Ｎ１）流感病毒血凝素（ＨＡ）基因核苷酸全序列进行了测定，其长度为１７７８ｂｐ，共编码５６６个氨基酸，其中信号区１７个，ＨＡ１区３２６个，多肽连接区１个，ＨＡ２区２２２个。与Ａ／ＮＪ／１１／７６（Ｈ１Ｎ１）毒株ＨＡ蛋白分子上氨基酸序列相比，其同源性为９４．５％，而ＨＡ１和ＨＡ２区的同源性分别为９３．６％和９７．７％。同时在ＨＡ１区的第２７６个位点上多１个糖基化点，然而其余的包括２个重叠的糖基化点均相同。The present paper describes the complete nucleotide and deduced amino acid sequences of the HA of A/Swine/Beijing/47/91 (H1N1) virus isolated from pigs in China The full-length of HA gene of isolate is 177bp encoding for 566 amino acids.There are 17 of them in signal region,326 in HA1 domain,1 in cleavage side between HA1 and HA2 domains,and 222 in HA2 area.The amino acid sequences on HA protein molecular of isolate compared with those of A/NJ/11/76(HN) virus.The homologies were 94.5% in HA,93.6% in HA1 and 97.7% in HA2,respectively.There was one more glycosylation site at 276 position on HA1 domain of isolate than that of A/NJ/11/76(H1N1) virus,but the other glycosylation sites including two overlaping glycosylation sequons all were same between the two viruses.(Institute of Virlolgy,Chinese Academy of Preventive Medicine,Beijing 100052)(St.Jude Children's Research Hospital,Memphis,TN,USA)

关 键 词：猪型 流感病毒 核苷酸类 血凝素 序列分析 

分 类 号：R373.13[医药卫生—病原生物学]