戊型肝炎病毒中国株结构区ORF2　cDNA的克隆及表达  

Cloning and Expression of the Open Reading Frame 2 cDNA from a Chinese Hepatitis E Virus Strain

作　　者：庄辉[1] 李凡[1] 朱永红[1] 朱万孚[1] 郭晓霞[1] 

机构地区：[1]北京医科大学流行病学教研室,澳大利亚墨尔本费菲医院维省传染病研究实验室,克法伦伯内特医学研究中心

出　　处：《中华实验和临床病毒学杂志》1995年第1期22-24,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：美国CMB资助

摘　　要：将成型肝炎病毒（ＨＥＶ）中国株结构区第二开放读码框架（ＯＲＦ２）８３７ｂｐｃＤＮＡ片段插入ｐＧＥＸ１λＴ质粒中进行表达。经免疫吸印法证明，该质粒表达的融合蛋白具有识别抗－ＨＥＶ的抗原表位，可用于制备抗－ＨＥＶ检测试剂。A 837 base cDNA fragment from the second open reading frame of the structual region of a Chinese HEV strain was inserted into the pGEX1λT expression plasmid.The Western blot assay indicated that the fusion protein expressed by this plasmid contains epitopes recongnized as anti-HEV antibodies.It demonstrates that this fusion protein is useful for the development of anti-HEV detection systems.

关 键 词：戊型肝炎病毒 CDNA 基因表达 基因克隆 疫苗 

分 类 号：R373.21[医药卫生—病原生物学] R392－33[医药卫生—基础医学]