胶体金联免疫吸附试验检测丙型肝炎病毒抗体  

Application of Gold Conjugated Immunosorbent Assay for Detection of Antibodies to Hepatitis C Virus

作　　者：皮国华[1] 何红英[1] 谷淑燕[1] 张素香[1] 邓瑶[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《中华实验和临床病毒学杂志》1995年第4期371-374,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：采用基因工程方法，用在大肠杆菌中表达的丙型肝炎病毒（ＨＣＶ）－Ｃｏｒｅ和ＮＳ３优势表位抗原，建立了检测ＨＣＶ－ＩｇＧ抗体的间接胶体金联免疫吸附试验。其特异性、敏感性均达到国内外ＥＬＩＳＡ试剂盒水平，其特点是操作简便，观察结果直观可靠，适于医院、血站和家庭自检。Purified core and NS3 antigens of hepatitis C virus(HCV) expressed in E. colt bacteria were cojugated with colloidal gold partides for detection of antibodies to HCV with the gold conjugated immunosorbent assay(GCISA). The specificity and sensitivity of this method were comparable to conventional ELISA. This method is spcific, sensitive, convenient and reliable as developed for detection of antibodies to various viral diseases. and may be useful in hospitals, blood transfusion facilities and even in families for self-diagnosis.

关 键 词：丙型肝炎病毒 抗体 ELISA 胶体金 

分 类 号：R512.630.4[医药卫生—内科学]