中国4株丁型肝炎病毒抗原编码区基因的cDNA克隆与序列分析  

Molecular Cloning and Sequencing and Comparison of Hepatitis D Antigen(HDAg)-Coding Fragment of Four Chinese Hepatitis Delta Virus Isolates

作　　者：谭文杰[1] 刘善虑[1] 苗季[1] 丛旭[1] 詹美云[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《中华实验和临床病毒学杂志》1995年第4期293-298,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：国家863高科技生物技术领域资助

摘　　要：从我国四川、广西、河南的丁型肝炎病毒抗原（ＨＤＡｇ）或抗体（ａｎｔｉ－ＨＤ）阳性的ＨＢｓＡｇ携带者中，筛选出４株ＨＤＶＲＮＡ阳性标本，经逆转录和聚合酶反应（ＲＴ－ＰＣＲ）后，获得包含ＨＤＶ抗原编码区的ＣＤＮＡ片段，并对其进行克隆与序列测定。经计算机分析比较表明：四川、广西株与美国－１株同源性最高，分别为９９．３％、９９．０％；而河南－１、河南－２株与台湾株的同源性较高。分别为９４．３％、９２．１％，上述４株与日本－１、秘鲁－１的同源性在６６．１％－７７．８％之间。推导的ＨＤＡｇ的氨基酸序列的同源性为：四川、广西株与美国株分别为９９．５％、９６．４％；河南－１、河南－２株与台湾株分别为８９．７％、８９．３％，故我国至少存在基因型Ｉ型的两种亚型，其中四川、广西株为ＩＡ型；河南２株皆为ＩＢ型。同时，实验结果还证实了ＨＤＶ毒株的异质性呈地区分布特征。Four hepatitis D virus RNA positive samples were selected from Sichuan, Guangxi and Henan provinces of China.The samples were HDAg or anti-HDAg positive by ELISA.All of them were collected from hepatitis B surface antigen (HBsAg)carriers. The cDNA fragments coding HDAg were obtained by means of reverse transcription and polymerase chain reaction.These fragments were cloned and sequenced. Comparison of thehomology of nucleotide (or amino)acid sequences of these Chinese isolates with other known HDV isolates showed that the homology isolates from Sichuan and Guangxi with those of US-1 strain(genotype IB) were about 99%(96%-99%),but the Henan-1,Henan-2 strains shared 92%-94%(about 89%)China.These HDV isolates also showed genetic and HDAg hetergencity and these may exist geographically.

关 键 词：丁型肝炎病毒 抗原 CDNA克隆 序列分析 

分 类 号：R373.2[医药卫生—病原生物学]