胆红素自由基对人胆汁沉淀过程的影响  

The effects of bilirubin free radical on the precipitation of human bile

作　　者：修典荣[1] 沈韬[1] 傅贤波[1] 林丛[1] 周孝思[1] 

机构地区：[1]北京医科大学第三医院外科

出　　处：《中华实验外科杂志》1995年第4期195-196,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金

摘　　要：胆红素用６０ＣＯ辐照后其所携带自由基强度增强（称ＢｉｌＥ），用甘露醇加ＶｉｔＣ洗涤其强度减弱（称ＢｉｌＲ），将ＢｉｌＥ和ＢｉｌＲ分别加入人胆汁中保温沉淀发现：ＢｉｌＥ管沉淀中糖蛋白（ＧＰ）、“胆红素钙”和总钙皆明显高于ＢｉｌＲ管。据此，我们认为胆红素自由基促进了糖蛋白和“胆红素钙”沉淀，有利于形成三元络合物，有促成石作用。In order to investigate the effect of bilirubin free radical on the bile precipitation,bilirubin reagent from the same batch was ra-diated by 60Co to enhance its free radical inten-sity(BilE)or treated with Vit C and mannitolto reduce its free radical intensity(BilR),andthe difference of intensity was confirmed withelectron paramagnetic resonance spectra. TheBilE and BilR were separately added into twoaliquots from a same bile supernatant.Thenthe paired bile samples were incubated for 36hrs,to form precipitate. Results :in the precipi-tate of tube BilE, bilirubin-calcium compound(B-Ca),glycoprotein and total ealcium weremore than those in tube BilR(P<0.05),butin supernatant,the concentrations of calcium,bilirubin and glycoprotein of BilE were lessthan those of BilR.So we consider that biliru-bin free radical was able to promote the pre- cipitation of B- Ca and glycoprotein , further- more, to induced their polmerization. Thesewere favourable to the formation of trinalcomplex composed of calcium bilirubinate andglycoprotein,the main ingredients of pigmentgallstones.

关 键 词：胆红素 自由基 胆色素 胆结石 病因学 

分 类 号：R657.420.2[医药卫生—外科学]