大鼠脾切除对免疫应答和调节的影响  

Effect of splenectomy on the immunologicresponse and immunoregulation

作　　者：成军[1] 陈易人[1] 陆云彪[1] 施从先[1] 

机构地区：[1]苏州医学院附属第一医院普外科,苏州医学院附属第一医院检验科,苏州医学院附属第一医院传染病研究室

出　　处：《中华实验外科杂志》1995年第6期356-357,共2页Chinese Journal of Experimental Surgery

摘　　要：对四组大鼠假手术、５０％脾切除、７５％脾切除和全脾切除后２、４和６周，腹腔注射绵羊红细胞，检测初级免疫应答的血清溶血素含量（ＯＤ值）、Ｔ细胞亚群及白细胞介素２受体（ＩＬ－２Ｒ）细胞。结果说明，脾切除（含７５％脾切）可减弱机体初级免疫应答，影响Ｔ细胞亚群，损害体液和细胞免疫。保留５０％脾脏可保留全部脾脏功能。The sequential immunologic consequencesand T lymphocyte subsets of total splenecto-my,and partial splenectomy were investigatedin an animal model.Adult male Wistar ratswere divided into four group:sham operation,50%splenectomy, 75%splenectomy and totalsplenectomy. At 2, 4 and 6 weeks followingoperation, animals in each group received asingle intraperitoneal injection of sheep redblood cells. Six days later, serum hemolysinOD values and T lymphocyte subpopulationswere measured. Results suggest that normalimmunologic function and T lymphocyte sub-sets could be achieved if approximately 50% of the spleen is conserved in situ;75%splenecto-my and total splenectomy do not normalize an-tibody responses; with approximately 25%ofsplenic tissue conserved in situs, antibody re-sponses; to intraperitoneal administered anti-gen may be improved with time. Meanwhile,rats with total and 75%splenotomy showed agreater increase in T suppressor cells (T_s)andgreater decrease in T helper cells(T_h)and T_h/T_scell ratio than those in sham operation and50%splenectomy groups(P<0.05).A com-pariso of Tn/T_s cell ratio between 4 weeksand 6 weeks after total splenectomy demon-strated significant increase of ratio in 6 weekgroup. The changes observed after splenecto-my indicate spleen plays a major role in im-munologic response and cellular immunoregu-lation.

关 键 词：脾切除 免疫应答 免疫调节 T细胞亚群 

分 类 号：R657.603[医药卫生—外科学]