一株新菌的发现及定位研究  

PROPOSAL OF A NEW GENUS FOR A BACTERIAL STRAIN ISOLATED FROM BLOOD OF A SEPTICEMIC PATIENT

作　　者：谷海瀛[1] 

机构地区：[1]吉林铁路中心医院检验科,132001

出　　处：《中华微生物学和免疫学杂志》1995年第2期92-94,共3页Chinese Journal of Microbiology and Immunology

摘　　要：从胆囊炎败血症患者血中分离出一株新菌,该菌为兼性厌氧G^-球菌,无鞭毛、无芽胞、无荚膜,发酵葡萄糖产气,还原硝酸盐成亚硝酸盐,氧化酶试验阴性,不具有氨基酸脱羧酶,对绝大多数糖不利用,具有β半乳糖苷酶,不产生吲哚,甲基红试验阳性,利用丙二酸盐,不利用枸橼酸盐。电镜表明该菌为芽殖细菌,G+C含量为47.58mol％。16S rRNA序列测定结果经计算机检索基因库所有序列进行比较表明和目前所有菌序列都不同,根据相似性和表型特征选择最接近的15个种16S rRNA序列得出全连锁聚类分析树状谱,亲缘关系相差甚远。结果表明该菌不属于已知的科、属,根据该菌发酵代谢和特有的芽殖特点,应建立新科。拟定为发酵芽殖菌科(Fermentobadaceae fam.nov.),命名Guhaiyingella gen.nov.guhaiyingii sp.nov。 该菌已收藏在中国微生物菌种保藏中心,CGMCC No.0205~T(T=type strain)。One bacterial strain isolated from the septicemic blood of a cholecystitis patient was identified as gram-negative cocci, facultatively anaerobic, non-motile and did not form spores and capsules. It grew well on MacConkey's media. Glucose was fermented with production of acid and gas. Acid was not produced form most other carbohydrates.It was oxidase-negative,weekly catalase-positive,indole-negative,lysine and ornithine decarboxylase negative,and reduced nitrates to nitrites. On electron micrographs its cells showed budding. Its G+C of DNA was 17.58 mol%(Tm). On the basis of 16S rRNA oligonucleotide similarity, dendrogram and average linkage clustering, this new bacterial strain did not fit well into any of the currently recognized categories. So,for this new strain,a new genus, Fermentobadaceae fam. nov. and Guhaiyingella gen. nov. Guhaiyingii sp. nov. was proposed. This type strain is CGMCC No. 0205T(T = type strain).

关 键 词：发酵芽殖菌 16SrRNA序列 胆囊炎 定位 

分 类 号：R372[医药卫生—病原生物学] Q939.109[医药卫生—基础医学]