应用多聚酶链扩增技术鉴别混合感染轮状病毒VP7的血清型别  

IDENTIFICATION OF VP7 SEROTYPES OF ROTAVIRUS COINFECT1ONS FROM STOOL SPECIMENS BY POLYMERASE CHAIN REACTION

作　　者：熊朝晖[1] 钱渊[1] 袁丽娟[1] 张又[1] 刘军[1] 关德华[2] 刘长青[3] 张振华[4] 赵慧欣[4] 王之梁[1] Vera Gouvea 

机构地区：[1]首都儿科研究所 [2]北京市儿童医院传染科 [3]长春市儿童医院 [4]首都儿科研究所附属儿童医院传染科 [5]Food and Drug Administration,USA

出　　处：《中华微生物学和免疫学杂志》1995年第2期105-109,共5页Chinese Journal of Microbiology and Immunology

摘　　要：1991年秋冬季收集了北京等我国四个地区婴幼儿腹泻患儿粪便标本,通过聚丙烯酰胺凝胶电泳(PAGE)发现一些标本中有不同轮状病毒的混合感染。应用套式多聚酶链反应(套式PCR),确定了7例混合感染轮状病毒的VP7血清型别,并用Northern印迹杂交法证实。该方法用于鉴别混合感染毒株的血清型别,不仅敏感、特异,而且节省时间和材料。Stool specimens from children with diarrhea were collected from four different areas of China in autumn and winter of 1991. The electrophoretic patterns of viral RNA on polyacrylamide gel suggested coinfection of rotaviruses in some of the specimens. The serotypes of rotavirus coinfections were determined by nested polymerase chain reaction,and were further confirmed by Northern blot hybridization.

关 键 词：婴幼儿腹泻 轮状病毒 套式 聚合酶链反应 

分 类 号：R446.5[医药卫生—诊断学]