原位PCR技术检测石蜡包埋肝组织中HBV DNA  

DETECTION OF HEPATITIS B VIRUS DNA IN LIVER TISSUES EMBEDDED IN PARAFFIN BY IN SITU POLYMERASE CHAIN REACTION

作　　者：刘玉兰[1] 杜绍财[1] 陶其敏[1] 

机构地区：[1]北京医科大学人民医院肝病研究所,100044

出　　处：《中华微生物学和免疫学杂志》1995年第2期140-142,共3页Chinese Journal of Microbiology and Immunology

摘　　要：采用原位PCR技术对22例石蜡包埋肝组织中HBV DNA进行检测,并与提取组织DNA PCR技术及原位杂交技术进行比较。结果表明,22例标本经原位PCR检测有9例为阳性;经提取组织DNA PCR检测有7例阳性,两者敏感性相近,符合率达80％;经原位杂交检测有4例阳性,其敏感性只及原位PCR的44％。提示原位PCR是项快速、敏感及特异性较高的检测技术。Twenty two slides of liver tissues hepatitis B virus DNA embedded in paraffin were detected by in situ polymerase chain reaction (ISPCR). and compared with the method of PCR by extracted DNA and in situ hybridization (ISH). The results showed that there were nine and seven positive slides by ISPCR and PCR, respectively. They had similar sensitivity. Only four positive slides were seen by ISH. It suggests that ISPCR might be a rapid,sensitive and specific technique method.

关 键 词：聚合酶链反应 ISPCR 原位杂交 肝细胞 

分 类 号：R446.1[医药卫生—诊断学]