丙型肝炎IgM的检测及对丙型肝炎患者血清的分析研究  

EXAMINATION OF IgM ANTIBODY TO HEPATITIS C AND ANALYSIS ON HEPATITIS C PATIENTS' SERA

作　　者：宁杨[1] 赵莉[1] 程明[1] 杨俊英[2] 陈乃玲 

机构地区：[1]卫生部北京生物制品研究所,100024 [2]解放军海军总医院 [3]解放军肝病研究所

出　　处：《中华微生物学和免疫学杂志》1995年第4期254-257,共4页Chinese Journal of Microbiology and Immunology

摘　　要：用丙型肝炎病毒(HCV)结构区(C区)和非结构区(Ms3区)制备固相抗原,建立了检测HCV IgM抗体的间接ELISA,并用此法检测了101份丙型肝炎病人血清。检测结果表明,急性输血后丙型肝炎病人和HCV抗体阳性伴ALT升高者,其抗C区IgM阳性者所占比例明显大于抗Ns3区。单独用C区抗原检测IgM抗体,其阳性率大于用C区和Ns3区抗原共同检测的结果。前者Cut off指数(COI)>2者占阳性例数的71.5％,后者仅占26％。因而显示了HCV结构区抗原在IgM检测中的重要意义以及抗C区IgM阳性有可能成为新近感染或活动性丙型肝炎的标志。A ELISA for testing IgM antibody to hepatitis C was developed with solid phase HCV structure antigen (core region) and nonstructure antigen (Ns3 region). 101 hepatitis C patients sera were tested. The results showed that positive ratio of IgM to core region (core positive/positive) was higher than that of IgM to Ns3 region (Ns3 positive/positive) in acute post-transfusion hepatitis C patients and in those anti-HCV positive with ALT-elevated pateints. Anti-HCV IgM positive ratio tested by core region antigen was higher than that by core region+ Ns3 region. The ratio of COI>2 accounted for 71. 5% among those positive tested by core region antigen and 26% among those tested by core region+ Ns3 region antigen. It was demonstrated that HCV structure antign was important in examination of IgM antibody to hepatitis C,anti-HCc IgM might be a marker indicating recent HC infection and perhaps also a marker for active HCV infection.

关 键 词：抗HCV IGM 丙型肝炎 CUT off指数 

分 类 号：R512.63[医药卫生—内科学]