再生障碍性贫血患者血浆对正常骨髓巨核系祖细胞体外增殖分化作用的研究  

THE EFFECTS OF PLASMA FROM APLASTIC ANEMIA PATIENTS ON PATIENTS ON PROLIFERATION AND DIFFERENTIATION OF BONE MARROW MEGKARYOCYTE PROGENITORS IN VITRO

作　　者：李震宇[1] 杨锦媛[1] 陈唤青[1] 陆德炎[1] 丁润生[1] 

机构地区：[1]南通医学院第一附属医院,镇江市第二人民医院

出　　处：《中华血液学杂志》1995年第8期408-410,共3页Chinese Journal of Hematology

摘　　要：应用骨髓长期培养（ＬＴＢＭＣ）法研究了再生障碍性贫血（再障）患者血浆的巨核细胞集落刺激活性（ＭＫ－ＣＳＡ）。分别用再障血浆、正常ＡＢ混合血浆和特发性血小板减少性紫癜（ＩＴＰ）患者血浆作为刺激正常骨髓巨核细胞生长的活性来源。结果显示三组培养贴壁层形成情况类似；再障组悬浮液中累积细胞数（３．１９±０．５４）×１０￣７高于对照组（２．４８±０．６０）×１０￣７，与ＩＴＰ组（２．６４±０．６４）×１０￣７比较，统计学差异不显著；再障组悬浮液中巨核系祖细胞（ＣＦＵ－ＭＫ）维持时间长。直接对正常骨髓细胞作半固体培养，发现再障组形成的巨核细胞集落数（９５．４±３５．２／２×１０￣５个细胞）高于对照组（４３．５±２０．６／２×１０￣５个细胞）。结果表明再障血浆具有较高ＭＫ－ＣＳＡ，推测再障患者可能存在着原发性ＣＦＵ－ＭＫ缺陷，血浆中ＭＫ－ＣＳＡ升高可能是机体的一种反馈性调节。y using long-term bone marrow culture(LTBMC )system,the megakaryocyte-colony stimulating activi-ties(MK-CSA) of the plasma from aplastic anemia(AA) patients on normal bone marrow cells were as-sayed and compared with those from patients with idio-pathic thrombocytopenic purpura(ITP)and normalcontrols(NC).The results showed that thestromalcell layer(SL) formations were similar in the threegroups,the number of nucleated cells in suspension ingroup AA(3.19 ±0.54)× 10￣7 was significantly higherthan that in control group(2.48 ±0.60)×10￣7,and theCFU-MK cultured with plasma from AA group sus-tained longer than that of the other two groups,In se-mi-solid culture of normal bone marrow cells.thenumber of MK colonies with AA plasma was also morethan that with control plasmas.

关 键 词：再生障碍性贫血 巨核细胞 集落刺激活性 血小板 

分 类 号：R556.502[医药卫生—血液循环系统疾病]