HLA－DPB_1等位基因与特发性血小板减少性紫癜的相关性研究  

ASSOCIATON BETWEEN HLA-DPB_1 GENE ANDIDOPATHC THROMBOCYTOPENIC PURPURA

作　　者：蒋惠源[1] 王晓冬[1] 储小红[1] 张威[1] 阮长耿[1] 

机构地区：[1]江苏省血液研究所,苏州医学院血栓与止血研究室,苏州医学院附属第一医院血液科

出　　处：《中华血液学杂志》1995年第3期118-121,共4页Chinese Journal of Hematology

基　　金：江苏省科委资助

摘　　要：采用多聚酶链反应（ＰＣＲ）结合限制性片段长度多态性（ＲＦＬＰ）技术对５３例正常人和５５例慢性特发性血小板减少性紫癜（ＩＴＰ）患者进行ＨＬＡ－ＤＰＢ＿１等位基因分型。结果显示，对照组无一例有ＤＰＢ＿１１３０１等位基因，ＩＴＰ组６例存在ＤＰＢ＿１１３０１等位基因（ＲＲ＝１４．０２，Ｐ＜０．０５），此外，ＤＰＢ＿１０２０１＋０２０２所对应的ＤＰ＿（ｗ２）抗原纯合子在ＩＴＰ组明显多于对照组（ＲＲ＝３．９４，Ｐ＜０．０１），而ＤＰ＿（ｗ２）杂合子明显少于对照组（Ｐ＜０．０２５），提示ＤＰＢ＿１１３０１等位基因及ＤＰ＿（ｗ２）抗原纯合子可能与ＩＴＰ的易感性有关，而ＤＰ＿（ｗ２）抗原杂合子可能具有保护作用。enomic HLA-DPB_1 typing of 53 unrelated Chi-nese blcod donors and 55 ITP patients was performed byusing polyrnerase chain reaction (PCR) followed by re-striction fragment length polymorphism (RFLP) analy-sis. The primers used in PCR were designed to anneal toflanking region of the second exon of DPB_1 gene . Allelicspecificity was determined by electrophoretic analysis ofthe pattern of restriction fragments which were generat-ed by digestion with several restriction endonucleases.The results showed that the frequencies of DPB_1 0501and DPB_1 0201 + 0202 allelles in Chinese were muchhigher, and the frequencies of DPB_1 and DPB_1 0401+0402 alleles were mere much lower than those in Cau-cacians. An increased frequency of DPB_1 1301 in pa-tients with ITP was revealed as compared with normalcontrols (RR =14. 02 , P<0. 05) . In addition , DPw_2homozygotes in ITP were much more than those in con-trols (P<0. 025). It suggested that DPB_1 1301 alleleand DPw_2 homozygosity may be associated with ITP-susceptibility , and DPw_2 heterozygosity may be protec-tive against the development of ITP.

关 键 词：紫癜 血小板减少 人白细胞抗原 多聚酶链反应 

分 类 号：R554.604[医药卫生—血液循环系统疾病]