儿童特发性血小板减少性紫癜骨髓巨核细胞成熟状态  

THE MATURATION OF MEGAKARYOCYTES FROMBONE MARROW IN CHILDREN WITH IDIOPATHICTHROMBOCYTOPENIC PURPURA (ITP)

作　　者：王同保[1] 张欣欣[1] 冯剑飞[1] 赵金枝[1] 申志强[1] 

机构地区：[1]河南省人民医院儿科,河南医科大学第三附属医院儿内科

出　　处：《中华血液学杂志》1995年第3期127-129,共3页Chinese Journal of Hematology

摘　　要：用血小板凝血酶敏感素（ＴＳＰ）、血小板膜糖蛋白（ＧＰ）Ⅱｂ／Ⅲａ复合物和ＩｇＧ单克隆抗体，用碱性磷酸酶－抗碱性磷酸酶染色法对７例正常儿童和２７例特发性血小板减少性紫癜（ＩＴＰ）患儿骨髓巨核细胞进行研究。结果发现：正常儿童巨核细胞ＴＳＰ和ＧＰⅡｂ／Ⅲａ含量与细胞成熟程度成正比；２７例患儿的巨核细胞ＧＰⅡｂ／Ⅲａ含量均降低，２３例ＴＳＰ含量减少，１２例巨核细胞上ＩｇＧ含量增高，且ＩｇＧ增高与ＴＳＰ降低有一定关系。提示，在儿童ＩＴＰ的发病过程中，不仅存在血小板破坏增加，而且巨核细胞的成熟受阻，这种受阻可能是血小板抗体作用于巨核细胞的结果。AbstractThe megakaryocytes (MK) from the bone marrow of27 children with ITP and 7 healthy donors with a panelof monoclonal antibodies against thrombospondin(TSP) , platelet glycoprotein (GP) Ⅱ b/Ⅲ a and im-munoglobulin (Ig) G by alkaline phosphatase anti-alka-line phosphatase (APAAP) methed. The results showedthat in normal children , the amounts of TSP and GP Ⅱb/Ⅲ a in MK were in direct proportion to the maturationof MK, and that among 27 patients with ITP, theamounts of TSP in 23 and the amounts of GPⅡ b/Ⅲa inall were decreased, and the amounts of IgG in 12 wereincreased. Moreover there was a certain relationship be-tween the increase of IgG and the decrease of TSP inMK. These changes demonstrate that in the pathogenet-ic preeess of ITP in children not only exists an increasedrate of platelet destruction, but also an obstruction ofmegakaryocyte maturation . The changes may be causedby antiplatclet antibody on MK.Key werde Megakaryocytes Alkaline phos-phatase anti-alkaline phosphataseThrombospondin

关 键 词：紫癜 血小板减少 巨核细胞 骨髓 碱性磷酸酶 

分 类 号：R554.6[医药卫生—血液循环系统疾病]