纤溶酶对血小板GPIb的作用  

EFFECT OF PLASMIN ON PLATELET GLYCOPROTEINIb

作　　者：黄惠民[1] 陈莹[1] 邱丽君[1] 史桂英[1] 

机构地区：[1]上海第二医科大学附属新华医院,上海第二医科大学生物物理教研室

出　　处：《中华血液学杂志》1995年第9期471-472,共2页Chinese Journal of Hematology

基　　金：国家自然科学基金

摘　　要：在洗涤血小板悬液中加入不同浓度纤维蛋白溶酶（纤溶酶）后，ＦＡＣＳ测得的抗ＧＰＩｂ单克隆抗体（单抗）的荧光结合强度随纤溶酶浓度的增加而逐渐下降，中止纤溶酶作用并无明显恢复。正常血小板经ＴｒｉｔｏｎＸ～１００处理后，抗ＧＰＩｂ单抗荧光结合强度明显增加。纤溶酶作用后的洗涤血小板无论是否经ＴｒｉｔｏｎＸ－１００处理，抗ＧＰＩｂ单抗荧光结合强度均明显减弱，但处理者为甚。ffect of plasmin on platelets glycoprotein Ib(GPIb)was studied with flow cytometer. The fluorescencebinding density of anti-GPIb monoclonal antibody(mAb) was reduced gradually with the plasmin con-centration increasing and there was no density restora-tion after plasmin effects were terminated and plateletswere incubated for one to three hours at 22℃. Thedensity of anti-GPIb mAb increased significantly whenplatelets were pretreated with Triton-100. After plas-min treatment , the density in both samples , Pretreatedor not with Triton-100, was reduced significantly

关 键 词：血小板膜糖蛋白 GPIB 纤维蛋白溶解酶 

分 类 号：R331.143[医药卫生—人体生理学]