脐血CD34^＋细胞体外扩增的研究  

IN VITRO EXPANSION OF UMBILICAL CORD BLOODCDs_(34)￣ + CELLS

作　　者：隋星卫[1] 张洪泉[1] 沈柏均[1] 

机构地区：[1]山东医科大学附属医院

出　　处：《中华血液学杂志》1995年第11期588-590,共3页Chinese Journal of Hematology

摘　　要：采用ＤｙｎａｌＭ－４５０ＣＤ＿（３４）免疫磁珠分离纯化脐血ＣＤ＿（３４）￣＋细胞，并探讨了不同细胞因子［干细胞因子（ＳＣＦ）、白细胞介素６（ＩＬ－６）、白细胞介素３（ＩＬ－３）及红细胞生成素（Ｅｐｏ）］组合对脐血ＣＤ＿（３４）￣＋细胞的体外培养和扩增作用。结果表明：①经ＤｙｎａｌＭ－４５０ＣＤ＿（３４）免疫磁珠分离的脐血ＣＤ＿（３４）￣＋细胞，其纯度达８５％～。９５％；②甲基纤维素培养体系中，在不同组合的细胞因子作用下，脐血ＣＤ＿（３４）￣＋细胞的集落产率明显不同，单用ＩＬ－６最低，ＳＣＦ、ＩＬ－６、ＩＬ－３及Ｅｐｏ联用产率最高；③在低氧液体培养体系中，ＩＬ－６和ＩＬ６＋Ｅｐｏ不能有效地扩增脐血ＣＤ＿（３４）￣＋细胞，而其它细胞因子组合均可产生明显的扩增作用；其中加Ｅｐｏ的组合扩增效果显著高于无Ｅｐｏ的相应组合。脐血ＣＤ＿（３４）￣＋细胞分离、纯化及其体外扩增的研究对于脐血造血细胞库的建立，脐血造血细胞移植和基因导入的研究均具有重要意义。D_(34)￣+ Cells from umbilical cord blood were purified by Dynalbeads M450 CD,. immunoselection system and cultured in the presence of various combina-tions of cytokines , including stem cell factor (SCF) .interleukin-3 (IL-3 ) , IL-6 and erythropoietin (Epo ).The results showed that OI 85%～ 95/00 of the cellsseparated by the immunobeads method were CD_(34) positive cells. ②In methylcellulose culture, the colonyyields of purified umbilical cord blood CD_(34)￣+ cells weredifferently stimulated by various cytokine combina-tions. IL-6 alone had the lowest colony yield, whilethe combination of SCF + IL-3 + IL-6 + Epo had thehighest colony yield. O3 In suspension culture, IL-6alone or IL6-Epo had little expanding effect on um-billical cord blood CD_(34)￣+ cells, while other cytokinecombinations could ecpand umbilical cord blood CD_(34)￣+cells at different levels. The expanding effects of thecombinations containing Epo were much superior totheir counterparts without Epo.The study of umbilical cord blood CD_(34)￣+ cells separation and their in vitro expansion open up the possibilityof the establishment of umbilical cord blood hematopoi-etic stemlprogenitor cells bank and clinical transplan-tation and gene transduction with these cells.

关 键 词：脐血 CD34^+细胞 细胞因子 体外扩增 

分 类 号：R446.112[医药卫生—诊断学]