依赖凝集素的人单核细胞介导的U937和Raji细胞系细胞程序性死亡  

LECTINDEPENDENT HUMAN MONOCYTEMEDIATEDAPOPTOSIS OF LEUKEMIC CELLS

作　　者：董海东[1] 邢嵘[1] 沈洁[1] 钱振超[1] 

机构地区：[1]大连医科大学病理生理学教研室,北京医科大学免疫系T细胞室

出　　处：《中华血液学杂志》1995年第12期636-638,共3页Chinese Journal of Hematology

基　　金：国家自然科学基金;青年基金

摘　　要：用ＤＮＡ电泳和荧光染色法流式细胞仪分析，发现在依赖美洲商陆凝集素（ＰＷＭ）的单核细胞介导的细胞毒活性中，白血病靶细胞Ｕ９３７和Ｒａｊｉ细胞均呈现以ＤＮＡ断裂为特征的细胞程序性死亡。通过细胞毒试验和荧光染色分析表明：抗肿瘤坏死因子α的单克隆抗体和乙酰基葡萄糖胺单糖可分别阻断单核细胞所诱导的Ｕ９３７细胞或Ｒａｊｉ细胞死亡和ＤＮＡ断裂。试验中还发现ＰＷＭ同时可与单核细胞和这两种肿瘤细胞相结合。提示凝集素在促进效靶细胞相互接近，进而导致肿瘤细胞发生程序性死亡方面具有重要作用。sing DNA electrophoresis and propidium iodide(PI)-DNA staining flow cytometry, we dernonstratedthat apoptosis of target U937 cells and Raji cells oc-curred in human monocyte-mediated cytotoxicity(LDMC) with the presence of pokeweed mrtogen(PWM). DNA fragmentation of U937 cells was inhib-ited by anti-TNFα monoclonal antibody tMcAb) . Byflow cytometry. we also found that PWM bound totumor cells as well as rnonocytes. These results sug-gest that the presence of lectinlike molecules on thesurfaces of monocytes and tumor cells might makethem close together to facilitate the induction of apop-tosis in leukemic cells.

关 键 词：单核细胞 凝集素 细胞凋亡 细胞程序化死亡 

分 类 号：R392.12[医药卫生—免疫学] R730.3[医药卫生—基础医学]