套式聚合酶链反应单管法检测单纯疱疹病毒  

Application of the nested polymerase chain reaction using a single tube method in berpse simplexvirus encephalitis

作　　者：金庆文[1] 侯熙德[1] 姚娟[1] 王宁山[1] 

机构地区：[1]南京医科大学第一附属医院神经内科

出　　处：《中华医学检验杂志》1995年第2期99-101,共3页

摘　　要：应用聚合酶链反应（ＰＣＲ）方法、套式ＰＣＲ双管法及套式ＰＣＲ单管法分别检测１３０份散发性脑炎患者脑脊液（ＣＳＦ）中单纯疱疹病毒（ＨＳＶ）的ＤＮＡ，阳性率依次为１８．５％（２４／１３０）、２９．２％（３８／１３０）、２９．２％（３８／１３０）；６０份非中枢神经系统感染者ＣＳＦ标本的检测阳性率分别为０、３．３％（２／６０）、０。结果显示：套式ＰＣＲ虽然提高了检测的敏感性，但假阳性也大大增加。改良的套式ＰＣＲ单管法则大大减少了污染机会，使操作更方便、省时，适于临床推广应用。30 CSF samples of sporadic encephalitis patients were examined to detect herpse simplex virusDNA by the different methods, including single polymerase chain reaction(PCR)assay, routine nestedPCR and newly designed single tube method. The positive detection rates were 18.5%(24/130 ),29.2%(38/130), 29.2%(38/130), respectively.When 60 CSF samples from the patients with no in-tracranial intection were compared,the positivities were 0%,3.3%(2/60),0%。 This results showedthat the positive detection rate of nested PCR was significantly higher, while the false positive rates in-creased. Recommended single tube method not only had the same positivity, but also reduced the risk ofcontamination. Single tube method would be more suitable for clinical usage.

关 键 词：单纯疱疹病毒 脑炎 套式 聚合酶链反应 

分 类 号：R373.11[医药卫生—病原生物学]