血浆D－二聚体检测及其临床应用  

Measurement of plasma D-dimer and its clinical application

作　　者：黄尉国 王鸿利[1] 张颖琪[1] 朱立红[1] 李耀辉[1] 王学锋[1] 程寅琳[1] 邵慧珍[1] 张鲁 葛维成 

机构地区：[1]上海市第七人民医院,上海第二医科大学瑞金医院,上海市捷门生物技术公司

出　　处：《中华医学检验杂志》1995年第2期71-74,共4页

摘　　要：利用自制单克隆抗体，建立酶联免疫吸附试验（ＥＬＩＳＡ）双抗体夹心法检测血浆Ｄ－二聚体含量，灵敏度达８ｎｇ／ｍｌ。检测３０例正常青壮年为０．１３±０．０３ｍｇ／Ｌ，１３例健康老年人为０．４４±０．１８ｍｇ／Ｌ二组比较差异有显著性意义（Ｐ＜０．０１）。检测５０例肝脏疾病、２８例急性粒细胞白血病、３５例急性心肌梗塞、６０例脑血管疾病和２２例外科手术的患者，结果血浆Ｄ－二聚体水平均较正常对照组升高（Ｐ＜０．００１）；同时急性心肌梗塞组和脑血管疾病组也较健康老年人组为高（Ｐ＜０．０１，Ｐ＜０．００１）。表明Ｄ－二聚体检测是一项鉴别原发性纤溶和继发性纤溶以及监测溶栓治疗的有价值的指标。he anthns measured the concentration of D-dimer in plasma by a sandwich-ELISA assay with two self-made McAbs.Its detection sensitivity was able to reach 8ng/ml.The average concentration of D-dimer in 30 normal adults was 0.13±0.03 mg/L, while that of D-dimer in 13 health olders was 0.44±0.18 mg/L,with a significant discrepancy between this two groups(P< 0.01).The investigation on 50 patients with liver diseases, 28 patients with acute graiiulocytic leukemia, 35 patients with acute my-ocardial infarction (AMI),60 patients with cerebrovascular diseases(CVD) and 22 patents after their surgical operations showed that the D-dimer levels in all of these persons were higher than those in con-trol group (P<0. 001),and that the D-dimer levels in AMI and CVD were higher than that in health older group(P<0.01,P<0.001 respectively)。

关 键 词：纤维蛋白 D-二聚体 血浆 临床应用 ELISA 

分 类 号：R446.61[医药卫生—诊断学]