胆红素氧化酶消除血清肌酐测定中胆红素的干扰  

Bilirubin oxidase method designed to minimize bilirubin interference in plasma creatinine assays

作　　者：陈伟英[1] 毛远丽[1] 陈小倩[1] 刘宏[1] 莫培生[1] 

机构地区：[1]解放军第三○二医院,卫生部临床检验中心

出　　处：《中华医学检验杂志》1995年第3期143-145,共3页

摘　　要：为消除肌酐测定中血清胆红素干扰，采用由卫生部临床检验中心提供的高纯度胆红素氧化酶（ＢＯＸ）和苦味酸试剂，设计了在ＣＯＢＡＳＦＡＲＡ－Ⅱ型全自动生化分析上检测肌酐的新方法，仅需样品８μｌ，ＢＯＸ８μｌ，苦味酸试剂８０μｌ，在１９０秒钟就可完成反应，所得结果表明，用该法对７５份总胆红素在０～６１６μｍｏｌ／Ｌ的标本进行检测，可以有效的消除胆红素对肌酐测定时的干扰。而且结果准确可靠，回收试验，黄疸血清标本用原法回收率仅为４９％～７１％，而用本法回收率可达９４％～１０５％。本法的重复性也较好，批内变异系数（ＣＶ）为２．８％～１２．７％，批间ＣＶ为１４．０％。本法操作简单，标本也不需要作人工预处理，是肌酐测定消除胆红素干扰比较理想的方法。he main interference while using Kinetic Jaffe’s method for the determination of creatinine was bilirubin;it made the results of creatinine inaccurate,lower and even negative in the samples of high bilirubin level.We selected high purity reagent of bilirubin oxidase(BOX) and ascorbatic acid,designed a new procedure on COBAS FARA-Ⅱ analvser to establish new method for determination of creatinine in jaundice patients. It only needs 8μl sample,BOX 8μl,Ascordatic acid 80μl and takes 190 seconds.The resul t was accurate,and indicated that the new method is free from interference or bilirubin. This delection method was used in 75 samples,which T Bil ranged from 0～616μmol/L. The result is accu-rate and reliable. The repetition is good.The CV of intra-group was 2.8%～12.7%,the CV of inter-group was 14.0%,The rate of recovery was 94%～105%. While the old method was 49%～71%. It is convenient to manipulate and the samples need no preparation. It is an idealistic method to determin creatinine deminishing bilirubin interference.

关 键 词：肌酐 胆红素 胆红素氧化酶 血清 干扰 

分 类 号：R446.112[医药卫生—诊断学]