血清钙的偶氮胂Ⅲ测定法  

Determination of calcium in serum with alkaline Arsenazo Ⅲ reagents

作　　者：刘倩予[1] 黄君富[1] 魏明竟[1] 

机构地区：[1]第三军医大学第一附属医院检验科

出　　处：《中华医学检验杂志》1995年第5期270-272,共3页

摘　　要：为了消除偶氮胂Ⅲ在酸性条件下测定血清钙所引起的蛋白质沉淀，报道一种在碱性条件下用偶氮脾Ⅲ测定血清钙的方法。在碱性条件下钙与偶氮胂Ⅲ的络合物在６５０ｎｍ波长的摩尔消光系数大大增加。加入８－羟基喹啉－５－磺酸可避免镁的干扰。在血清胆红素高达５００μＬｍｏｌ／Ｌ、血红蛋白１２ｇ／Ｌ及脂肪乳剂Ｉｎｔｒａｌｉｐｉｄ高达１０ｇ／Ｌ时，均对本法无显著干扰。钙标准曲线在０～５．０ｍｍｏｌ／Ｌ范围内线性良好。该法与邻甲酚酞络合酮测定法所得结果相关性好。We propose a method for determination of calcium in serum using Arsenazo Ⅲ in alkaline medium. In the alkaline medium,the molar absorptivity of the Arsenazo Ⅲ complex with calcium at 650nm in-creases significantly. The interference from magnesium is avoided by using the 8-HQS. The assay showed no interference from as much as 5 00μmol/L of bilirubin,12g of haemoglobin and 10g of in-tralipid per liter. The standard curve for calcium was linear from 0 to 5.0mmol/L. The results from proposed method showed good agreement with results by OCPC procedure.

关 键 词：钙 偶氮胂III 比色法 

分 类 号：R446.112[医药卫生—诊断学]