东莨菪碱对吗啡成瘾大鼠下丘脑、垂体及血β内啡肽和催产素含量的影响  

Effect of scopolamine on the contents of β-endorphinand oxytocin in hypothalamus, pituitary and plasma inmorphine dependent rats

作　　者：杨国栋[1] 顾钧[1] 叶明珠[1] 唐甩恩[1] 

机构地区：[1]宁波市微循环与莨菪类药研究所

出　　处：《中华医学杂志》1995年第1期8-10,共3页National Medical Journal of China

摘　　要：用放射免疫法测定了东莨菪碱对吗啡成瘾大鼠下丘脑、垂体和血浆β内啡肽样免疫活性物质（ｉｒ－β－ＥＰ）和催产素样免疫活性物质（ｉｒ－ＯＴ）含量的变化。结果表明：（１）吗啡成瘾后，大鼠下丘脑、血浆中ｉｒ－β－ＥＰ和ｉｒ－ＯＴ含量增加（Ｐ＜０．０１），而垂体中ｉｒ－β－ＥＰ和ｉｒ－ＯＴ含量降低（Ｐ＜０．０１），提示大鼠脑内β－ＥＰ和ＯＴ含量的改变可能与吗啡的依赖和耐受产生有关。（２）东莨菪碱治疗３天和４天后，下丘脑中ｉｒ－β－ＥＰ含量增加（Ｐ＜０．０１），但ｉｒ－ＯＴ含量降低（Ｐ＜０．０１），垂体中ｉｒ－β－ＥＰ和ｉｒ－ＯＴ含量增加（Ｐ＜０．０１），提示东莨菪碱可能通过改变大鼠脑内下丘脑－垂体轴β－ＥＰ和ＯＴ的含量从而减轻对吗啡的依赖和耐受的产生。ale Sprague-Dawley rats weighing 180 ～ 220gwere rendered dependent on morphine by repeated injec-tions of morphine in increasing doses for 14 days. 0. 3mg/kg of scopolamine was injected intraperitoneally bidfor 3 and 4 days. Control rats were similarly injectedwith saline. The contents of β-endophin (β-EP) andoxytocin (OT) in hypothalamus, pituitary and plasmawere measured by radioimmunoassay. The resultsshowed that both β-EP and OT in hypothalamus andplasma increased but both were decreased in pituitary inmorphine dependent rats (P<0.01) . After scopolaminetreatment, the contents of β-EP increased but OT de-creased in hypothalamus (P<0. 01) , and both elevatedsignificantly in pituitary(P< 0.01). The results sug-gested that scopolamine might modulate hypothalamus-pituitary system to affect the realease or synthesis of β-EP and OT in the brain.

关 键 词：下丘脑 东莨菪碱 吗啡瘾 内啡肽类 戒毒 

分 类 号：R595.405[医药卫生—内科学]