原位杂交检测人动脉粥样硬化斑块组织中人巨细胞病毒DNA  

Detection of HCMV DNA in vascular plaques of atherosclerosis by in situ hybridization

作　　者：陈瑞珍[1] 李武[1] 杨英珍[1] 

机构地区：[1]上海医科大学中山医院上海市心血管病研究所

出　　处：《中华医学杂志》1995年第10期592-593,共2页National Medical Journal of China

摘　　要：为进一步探讨人巨细胞病毒（ＨＣＭＶ）在动脉组织及细胞中的存在状态，分布情况以及ＨＣＭＶ感染在动脉粥样硬化改变中的作用。作者以生物素标记的ＨＣＭＶＤＮＡ探针作原位杂交，检测了３２例临床确诊为动脉粥样硬化患者的动脉血管组织以及３２例患者的正常血管组织（２２例脑外伤死亡患者取动脉，１０例动脉硬化患者于搭桥术时取大隐静脉组织）中ＨＣＭＶＤＮＡ序列。结果其阳性分别为１３／３２及３／２２，表明ＨＣＭＶ感染与动脉粥样硬化的发生密切相关。在ＨＣＭＶＤＮＡ阳性标本中，ＨＣＭＶＤＮＡ主要见于血管内皮，内膜下平滑肌及中膜平滑肌，并多集聚于细胞核中，外膜组织及细胞外间质则少见。提示ＨＣＭＶ感染可能参与动脉粥样硬化的发生发展过程。To examine the possible role of HCMV in the etiology of atherosclerosis, we searched for the presence of HCMV DNA in arterial vascular tissues by in situ hybridization. Biotin labelled HCMV DNA probe was used on specimens of arterial wall taken from patients with clinically significant atherosclerosis. Evidence for the presence of HCMV DNA was obtained in 13 specimens out of 32 atherosclerosis specimens. In contrast, only 1 specimen was positive in the control group (Total 15 specimens of normal arterial and venous wall) , suggesting that there is a close relationship between human cytomegalovirus infection and atherosclerosis development. The results also showed that the positive hybridization signals were mainly observed in the nuclei of smooth muscular cells, rarely found in outer layer of arterial walls, cell membrane and cytoplasm. It was indicating that HCMV infection might be an etiological agent in the development of atherosclerosis.

关 键 词：动脉粥样硬化 巨细胞病毒 原位杂交 

分 类 号：R543.502[医药卫生—心血管疾病]