花粉中微管与花粉管伸长及其胞质颗粒运动的关系  

The Relation of Microtubules in Pollens to Pollen Tubes Growth and Their Cytoplasmic Particle Movements

作　　者：王秀珍[1] 韩志强[1] 

机构地区：[1]北京农业大学生物化学实验室,北京100094

出　　处：《作物学报》1995年第2期150-154,共5页Acta Agronomica Sinica

基　　金：国家自然科学基金资助课题

摘　　要：采用SDS-PAGE分离花粉中的微管蛋白。并用间接免疫荧光技术观察到花粉管中存在微管,这些微管呈束状形式沿花粉管伸长方向分布。 花粉培养在含不同微管解聚剂的培养液中,发现这些药剂对花粉萌发均有抑制作用,但其抑制的有效浓度不同。以植物微管特效解聚剂甲基氨草磷（Amiprophos-methyl APM）抑制的有效浓度为最低。用APM处理已萌发的花粉,还发现它能减缓花粉管中胞质颗粒运动的速度,甚至使运动停止。但这种抑制是可逆的。以上结果表明花粉管中存在微管,它与花粉萌发、花粉管伸长及其胞质颗粒运动均有密切的关系。The tubulin in pollens of maize was separated by SDS-PAGE and the microtubules of pollen tubes were observed by utilizing indirect immunofluorescence. It was found that the microtubules were boundles-shaped and distributed in the same direction as the elongation of the pollen tubes.Pollens were cultivated in medium solutions with different microtubule-depolymerizing agents. It was discovered that all these agents could inhibit pollen permination, but their effective concentration is quite different. When the pollen tubes were treated with APM-a microtubule specific inhibitor, the cytoplasmic particle movement began to slow down and finally stopped. But the inhibition is somewhat reversible. These results suggest that microtubules did exist in pollen tubes, and they are closely related with pollen tubes growth and cytoplasmic particle movements.

关 键 词：微管 花粉 甲基氨草磷 胞质颗粒运动 

分 类 号：Q944.58[生物学—植物学]