EHF病毒在人肾细胞融合中的结构蛋白分子定位分析和人胚肾细胞融合的电镜观察  

DETECTION AND LOCALIZATION OF HANTAVIRUS STRUCTURAL PROTEIN EXPRESSED ON THE SURFACE OF THE FUSION CELLS OF HUMAN KIDNEY AND OBSERVATION BY ELECTROMICROSCOPY ON THE FUSION CELLS OF HUMAN EMBRYO KIDNEY

作　　者：马立宪 邵丽华 吕敏和 李伯勤 赵丽 吕涛 吴世英 王子骥 

出　　处：《山东医科大学学报》1995年第2期122-126,共5页Acta Academiae Medicinae Shandong

摘　　要：用辣根过氧化物酶标记ＭｃＡｂＦ（ａｂ＇）_２、ＭｃＡｂＬＶ４８Ａ、ＭｃＡｂ３Ｄ_８和ＭｃＡｂＡ_（３５），分别对２７例ＥＨＦ患者尿液中融合细胞（ＦＣ）进行免疫酶染色。结果ＭｃＡｂＦ（ａｂ＇）_２检出率为２７／２７（１００％）；ＭｃＡｂＬＶ４８Ａ为１９／２７（７０．３７％）；ＭｃＡｂ３Ｄ_８为２０／２７（７４．０７％）；ＭｃＡｂＡ_（３５）为２／２７（７．４１％）。人胚肾细胞感染ＥＨＦ病毒后，在偏酸性（ｐＨ６．０）条件下可以产生特异性细胞融合现象，并经电镜证实了细胞之间膜的融合。此结果表明ＥＨＦ病毒可以导致人肾细胞融合，ＥＨＦ患者尿液中ＦＣ的形成可能与ＥＨＦ病毒膜蛋白在宿主细胞膜上的表达有关。Using McAb immunoperoxidase technique, 27 cases of EHF patients urine, in which the fusion cells (FC ) had been found,were detected and Hantavirus antigens were found on or in all the FC.At the same time, for localizing the structural proteins of Hantavirus (such as 48kd nucleocapsid protein NP, and two envelope glycoproteins (G1 68kd,G2 55kd ) ,LV48A(McAb direct against the G2 of A9 strain),3 D8 ( McAb against the hemagglutinin of Chen strain ) , and A35 (McAb against NP of A9 strain ) were used in detecting 27 cases of FC. The result ahowed that 19 presented positive reaction to LV48A,20 to 3D8and 2 to A35.This result indicated that the formation of the FC should be closely related to the Hantavirus glycoproteins, or hemagglutinin expressed on the membrane of host cells.After human embryo kidney cells (HEKC ) were infected by hantavirus, the cell fusion was found to occur under acidic conditions (pH6.0 ) .Under electromicroscope, the cell fusion was confirmed.

关 键 词：尿 细胞融合 流行性出血热 电子显微镜 

分 类 号：R512.8[医药卫生—内科学] R512.8[医药卫生—临床医学]