二苯甲烷二异氰酸酯作业工人血清中抗原特异性IgG抗体测定  

SPECIFIC SERUM IgG ANTIBODIES AGAINST DIPHENYLMETHANE DIISOCYANATE IN OCCUPATIONALLY EXPOSED WORKERS

作　　者：文保元 刘风华 李琦[1] 马瑞霞[1] 徐孝华[1] 

机构地区：[1]山东省劳动卫生职业病防治研究所,济南市职业病防治院

出　　处：《山东医科大学学报》1995年第2期130-133,共4页Acta Academiae Medicinae Shandong

摘　　要：观察２５例二苯甲烷二异氰酸酯（ＭＤＩ）作业工人、８例ＭＤＩ哮喘患者和３０例正常非接触者的血中抗原特异性ＩｇＧ水平，用ＭＤＩ－ＨＳＡ（人血清白蛋白）作抗原，ＥＬＩＳＡ法测定特异性ＩｇＧ抗体，三组的ＯＤ均值分别为０．５１±０．３１、０．７３±０．２９和０．２７±０．１０，阳性率分别为４４％（１１例）、８７．５％（７例）和６．７％（２例）。表明ＭＤＩ接触者血清特异性ＩｇＧ水平明显增高，且与呼吸道症状高度相关（ｒ＝０．８８，Ｐ＜０．００１），此指标可作为ＭＤＩ的接触标志和ＭＤＩ哮喘的病因诊断依据。Specific serum IgG antibodies against Diphenylmethane Diisocyanate in occupationally exposed workers was determined in twenty-five exposed workers and eight Patients with MDI-asthma, thirty non-exposed subjects as control groups.ELISA method was used by MDI-human serum albumin (HSA) conjugate. The average OD value of the three groups was 0.51 ±0.31, 0.73±0.29 and 0.27±0.10,respectively. The positive rates were 44% (11/25), 87.5%(7/8) and 6.7%(2/30) respectively in the three groups. The data demonstrated that specific IgG level was significantly increased in heavily exposed workers.There was high correlation between specific IgG level and respiratory symptoms (r=0.88, P<0.001). The specific IgG assay in vitro can be used as an indication of exposure to MDI and as a basis of etiological diagnosis to MDI-induced asthmatic patient .

关 键 词：免疫球蛋白G 哮喘 MDI 免疫酶联测定法 

分 类 号：R446.62[医药卫生—诊断学]