芦笋提取物对DNA拓扑异构酶Ⅱ活性作用的研究  

EFFECTS OF EXTRACTUM OF ASPARAGUS OFFICINALIS ON TOPOISOMERASE Ⅱ ACTIVITY

作　　者：曲显俊[1] 崔淑香[1] 左春旭[1] 

机构地区：[1]山东省医学科学院药物研究所

出　　处：《山东医科大学学报》1995年第2期165-167,共3页Acta Academiae Medicinae Shandong

摘　　要：通过体外ＤＮＡ拓扑异构酶Ⅱ介导ＤＮＡ裂解实验观察了芦笋提取物对该酶活性的影响。从小鼠白血病Ｌ１２１０细胞中提取ＤＮＡ拓扑异构酶Ⅱ，以ｐＢＲ（３２２）ＤＮＡ为底物，将１μｇＤＮＡ与０．２Ｕ拓扑异构酶Ⅱ温孵，当分别加入芦笋提取物２２０、２２、２．２μｇ／ｍｌ３个终浓度时，裂解能力可提高到原来的５倍，说明该药的直接作用靶点是ＤＮＡ拓扑异构酶Ⅱ，而不是直接裂解ＤＮＡ。The purpose of the test is to investigate the principle of activity of Extractum of Asparagus Officinalis promoting DNA topoisomerase Ⅱ activity of cleaving DNA. DNA topoisomerase Ⅱwas isolated from mouse leukemia L1210 cells and took PBR322 DNA as the substrates. After incubation of pBR322 DNA 1μg with 0.2U of topoisomerase Ⅱ, the presence of Extractum of Asparagus Officinalis by 220;22; 2.2μg/ml respectively, it reproducible stimulated DNA cleavage by 5-fold. It may be the fact that DNA topoisomerase Ⅱ is a direct target in antitumor activity of Extractum of Asparagus Officinalis.

关 键 词：芦笋提取物 DNA 拓扑异构酶II 酶活性 

分 类 号：R965.2[医药卫生—药理学]