免疫组化和核酸斑点杂交技术检测女性下生殖道人乳头状瘤病毒  

DETEGTON OF HUMAN PAPILLOMAVIRUS IN FEMALE LOWER GENITAL TRACT BY IMMUNOHISTOCHEMICAL STUDY AND DOT-BLOT HYBRIDIZATION TECHNIQUE

作　　者：陆惠娟[1] 郭德宏[1] 王明雁[1] 丁爱华[1] 张幼辰[1] 钱荆[1] 

机构地区：[1]上海医科大学附属妇产科医院,上海医科大学预防医学研究所

出　　处：《上海医学》1995年第9期504-507,共4页Shanghai Medical Journal

摘　　要：我院１９９２年７月～１９９３年３月共４９５份病理诊断为湿疣（５２份）、鳞状乳头状瘤病（２０２份）和非特异性炎或增生性病变（２４１份）的外阴、阴道及宫颈赘生物的活检标本，分别采用免疫组化ＡＢＣ法和核酸斑点杂交技术检测人乳头状瘤病毒的衣壳抗原和ＤＮＡ。在湿疣、鳞状乳头状瘤病和非特异性炎或增生性病变中，ＨＰＶ－Ａｇ阳性检出率分别为６９．２３％（３６／５２）、０．００％和０．００％；ＨＰＶ－ＤＮＡ阳性检出率分别为８４．６２％（４４／５２）、１１．３８％（２３／２０２）和３．３１％（８１／２４１）。免疫组化法和核酸斑点杂交检测湿疣和鳞状乳头状瘤病中ＨＰＶ的阳性率均有极显著性差异（Ｐ＜０．０５）。因此，认为湿疣与鳞状乳头状瘤病是二种不同的疾病。ＨＰＶ在鳞状乳头状瘤病和非特异性炎或增生性病变中的阳性率也有极显著性差异，推测鳞状乳头状瘤病者易受ＨＰＶ感染，对其可予对症处理并随访。免疫组化ＡＢＣ法检测ＨＰＶ的阳性结果可靠，定位清晰，有助于组织学表现不典型湿疣的诊断。核酸斑点杂交技术敏感，可为感染病毒分型提供依据。Four hundred ninety-five specimens,biopsied during July 1992 to March 1993,pathologically diagncsed to be vulvar, vaginal or cervical condylomas in 52,squamouspapillomatosis in 202, non-specific inflammation or hyperplastic change in 241,werescreened for detection of human papillomavirus(HPV)-Ag or HPV- DNA by immuno-histochemical study and dot-blot hybridization technique.During immunohistochemicalstudy,HPV-Ag positivity was observed in 36(69.23%)out of 52 samples of condylo-ma and none out of all samples of squamous papillomatosis and non-specific inflam-mation or hyperplastic change. HPV-DNA positivity was observed in 44(84.62%)outof 52 samples of condyloma,in 23(11.38%)out of 202 samples of squamous papillo-matosis and in 8(3.31%)out of 241 samples of nonspecific inflammation or hyperpla- stic change.The statistical analysis showed a significant difference between condylomaand squamous papillomatosisP< 0. 005),and between squamous papillomatosis andnon-specific inflammation or hyperplastic change (P< 0. 005).Squamous papillomatosismay be regarded as a different entity from condyloma, Women with squamous papillo- matosis were easily infected by HPV. Treatment should be individualized according tothe clinical situation. The positivity of detecting HPV by immunohistochemical study was reliable,andthe localization was clear. Dot-blot hybridization technique was sensitive,and could beused for subtyping of virus.

关 键 词：人乳头状瘤病毒 核酸斑点杂交 免疫组织化学 

分 类 号：R446.5[医药卫生—诊断学] R730.261[医药卫生—临床医学]