大肠杆菌新菌毛抗原的研究V．用标记抗体技术检验菌毛抗原  

STUDY ON THE NEW FIMBRIAL ANTIGEN OF E.coli V.EXAMINATION ON THE FIMBRIAL ANTIGEN USING LABELLED ANTIBODY TECHNOLOGY

作　　者：房海[1] 陈翠珍[1] 王廷富[1] 田在滋[1] 王洪发[1] 汤生玲[1] 

机构地区：[1]河北农业技术师范学院动物科学系

出　　处：《河北农业技术师范学院学报》1995年第3期1-5,共5页

基　　金：国家自然科学基金

摘　　要：应用荧光抗体染色、免疫酶染色及斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）对大肠杆菌Ｆ１９８７新菌毛抗原进行了检验，经可行性、特异性及对粪便标本检验等试验，表明这些方法均可用于对Ｆ１９８７新菌毛抗原的检出。三种方法除均表现特异、准确外，又分别具有各自的特点。荧光抗体染色结果易分辨，但需具备荧光显微镜；免疫酶染色只需普通显微镜；Ｄｏｔ－ＥＬＩＳＡ可用眼观判定结果，但对病料标本需做一定的处理后才能进行检验。本次试验均采用了间接染色法，同时试验建立了三种方法对Ｆ１９８７新菌毛抗原的具体操作程序。The new fimbrial antigen of E. coli was examinated,by using fluorescent antibody tech-nique ,immunoenzyme staining and Dot-ELISA. The result of feasible ,specificity and stoolexamination showed that all the mathods used above could examine F1987 new finbrial anti-gen. The three methds expressed not only specific ,accurate and each had own characteris-tics. By fluorescent antibody staining ,the result was easy to distinguish,but it needed fluo-rescence microscope;by immunoenzyme staining ,it only needed ordinary microscope and byDot-ELISA the results could be seen by naked-eye but it should be handled after specialtreatment of the specimen. This experiment used indirect fluorescent antibody staining. Thetest set up three methods of working procedure to F1987 new fombrial antigen.

关 键 词：家畜 大肠杆菌 新菌毛抗原 标记抗体 检验 

分 类 号：S852.61[农业科学—基础兽医学]