淋巴细胞经TCR－CD3活化增殖作用的分析  

Study of Lymphocyte Activation and DNA Synthesis via CD3-TCRComplex

作　　者：李鸣[1] 杨敬[1] 沈关心[1] 张茜[1] 刘忠北 刘慎沛[1] 叶维新[1] 

机构地区：[1]同济医科大学基础医学院免疫学教研室,郑州市空军医院检验科,同济医科大学实验医学研究中心核医学研究室

出　　处：《免疫学杂志》1995年第2期91-94,共4页Immunological Journal

摘　　要：本文探讨了抗ＣＤ３单抗诱导的淋巴细胞活化增殖及有关影响因素。实验结果表明：①淋巴细胞内钙升高是淋巴细胞活化增殖的重要条件，ＣＤ３ＭｃＡｂ引起的早期胞浆游离钙迅速升高主要由内质网释放钙离子所致，而淋巴细胞增殖不仅需要细胞内钙释放，还需要细胞外钙内流；②ＧＴＰ结合蛋白是淋巴细胞激活过程的一重要环节，经Ｇ蛋白作用物霍乱毒素作用后，淋巴细胞ＤＮＡ合成显著降低；③新霉素和ＰＳＳ可抑制ＰＬＣ和ＰｋＣ的活性，对淋巴细胞ＮＤＡ合成造成剂量依赖性抑制作用。此外，抗ＣＤ３ＭｃＡｂ诱导的淋巴细胞ＤＮＡ合成需要辅佐细胞的存在，高度纯化的Ｔ细胞对ＣＤ３ＭｃＡｂ的刺激不发生增殖反应。T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyteDNA synthesis in vitro. Several interference factors were discussed.The result indicated that lymphocyte acti-vation and proliferation is calcium dependent.A rise of cytoplasmic free Ca￣(2+) quickly following activation withCD3-McAb is due to intracelluar mobilization of Ca￣(2+),while lymphocyte proliferation needs both intracellularmobilization of Ca￣(2+)as well as influx of extracellular Ca ￣(2+). It is comfirmed that CTX sensitive G protein playsa role in regulating T cell proliferation by pretreatment CTX suppressing lymphocyte ￣3H-TdR incorporationobviously.PLC and PKC inhibitor,Neomycin and P.S.S,could also decrease T cell proliferation respectively.Lymphocyte DNA synthesis induced by anti-CD3 McAb depends on existence of accessary cells. Hightly puri-fied T cells didn￣’t respond to anti-CD3 McAb￣’s stimulation.

关 键 词：CD3McAb 淋巴细胞活化增殖 G蛋白 胞浆游离钙 

分 类 号：R392.12[医药卫生—免疫学]