慢性常压缺氧和缺氧伴CO_2潴留大鼠红细胞变形能力与膜收缩蛋白的研究  

Study on erythrocyte deformability and spectrin in rat during chronic isobaric hypoxia and CO_2 retention

作　　者：杨晓静[1] 钱桂生[1] 毛宝龄[1] 黄永平[1] 

机构地区：[1]重庆第三军医大学新桥医院呼吸中心

出　　处：《中国病理生理杂志》1995年第4期404-408,共5页Chinese Journal of Pathophysiology

摘　　要：本文测定了慢性常压缺氧（Ⅱ组）、缺氧伴ＣＯ－２潴留（Ⅲ组）及对照组（Ⅰ组）大鼠红细胞变形能力与红细胞膜收缩蛋白二聚体（ＳＰ－Ｄ）和四聚体（ＳＰ－Ｔ）的相对含量。结果表明，Ⅱ、Ⅲ组红细胞变形指数（ＤＩ）和ＳＰ－Ｄ、ＳＰ－Ｔ相对含量与Ⅰ组均有显著差异，且Ⅱ、Ⅲ组的ＤＩ与ＳＰ－Ｄ／ＳＰ－Ｔ比值呈显著负相关。提示膜收缩蛋白的异常，可能是导致慢性缺氧和伴ＣＯ－２潴留大鼠红细胞变形能力降低的重要因素之一。Animals were divided into 3 groups,i.e.chronicisobaric hypoxia(group Ⅱ);chronic isobaric hypoxia and CO_2 retention(groupⅢ)and control group,groupⅠ( CG). Changes of erythrocyte deformability and spectrin dimersSp-D)and tetramers(Sp-T)were investigated.The results demonstrated that:theerythrocyte deformation index and the relative content of Sp-T were significantly lower in group ⅡandⅢ than that in CG.The relative content of Sp-D and the ratio of Sp-D/p-Twere significantly higher in group Ⅱ andⅢ than that in CG.The erythrocyte deform-ability in group Ⅱ andⅢ were inversely related to the ratio of Sp-D/Sp-T. Thesefindings suggested that the abnormality of spectrin may be one of the reasons that re-Sulted in erythrocyte deformability decrease in rat during chronic isobaric hypoxia and CO_2 retention.

关 键 词：红细胞 膜收缩蛋白类 缺氧症 二氧化碳潴留 大鼠 

分 类 号：R594.302[医药卫生—内科学] R363.13[医药卫生—临床医学]