中国北方汉族人群HLA-DQα基因型及其等位基因频率的分布  

A STUDY ON THE DISTRIBUTION OF THE HLA-DQα ALLELE AND GENOTYPE IN NORTHERN CHINESE HAN POPULATION

作　　者：姜先华 吕世惠 阎威 王军 和中年 朱郁文 王国林 韩景松 魏平 

机构地区：[1]辽宁省刑事科学技术研究所,沈阳110032

出　　处：《中国法医学杂志》1995年第1期1-4,共4页Chinese Journal of Forensic Medicine

摘　　要：应用PCR和等位基因特异性寡该苷酸（ASO）探针杂交技术对中国北方汉族人群的HLA－DQα基因进行分型。在246例无关个体中观察到4种等位基因组成的10种基因型。等位基因频率分布在10．0～31．9％之间。基因型的分布符合Hardy－Weinberg定律。DP值为0．8669。对6个家系49个个体的调查表明，HLA－DQα基因按孟德尔方式遗传。不同人群HLA－DQα的DP值比较，中国人群高于其它人群。In this paper, the polymerase chain reaction(PCR) and allele sedic oligonucleoide (ASO) hybridization were employed to detect frequencies of the HLA-DQα alleles and genotypes of the northern Chinese Han population. Ten genotypes determined by four alleles of HLA-DQα lori were observed among 246 unrelated individuals with allele frequencies ranging from 10. 0% to 39. 1 %. The distribution of the observed genotypes is in Hardy-Weinberg equilibrium. The discrimnating power(DP) of this system in 0. 8669. A study of six families having 49 individuals demonstrated that the inheritance of the HLA-DQα genes is in Mendelian fashion. Additionally, the data of this study was compared with the results of the PCR-ASO-based genotyping of HLA-DQα loci of various populations which had been reported previously.

关 键 词：HLA-DQa基因 聚合酶链反应 ASO 群体遗传学 

分 类 号：R89[医药卫生—法医学]