pYNZ22位点扩增片段长度多态性法医应用的研究  

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF pYNZ22 LOCUS IN 120 CHINESES AND ITS APPLIFICATION TO FORENSIC SCIENCE PRACTICE

作　　者：胡兰 倪锦堂 陈松 季安全 叶健 李伯龄 

机构地区：[1]公安部第二研究所,北京100038

出　　处：《中国法医学杂志》1995年第1期5-7,共3页Chinese Journal of Forensic Medicine

摘　　要：对120名中国人进行pYNZ22位点扩增、银染检测，已找到11个等位基因，频率分布于0.4～30.4％，片段长170bp～870bp，相邻等位基因长度相差70bp，杂合度为73％，DP值为0．938。分析了五个家系均符合孟德尔定律。同一个体的血液、血斑、精液、混合斑中分离出的精子、毛囊、唾液及其它有核细胞组织的DNA多次进行pYNZ22位点扩增，得到一致的结果。灵敏度达到0．5ng核DNA。A region of the D17S30(pYNZ22) locus was amplified in DNA from 120 unrelated chinese individuals was carried out. The amplified products were analysed by mini-polyacrylamide gel electrophoresis followed by silver stain. 11 alleles, ranging from 170bp to 870bp in size and from 0. 4 % to 30. 4 % in frequency, was detected. The heterozygosity was 73%, Dp value was 0.938. The family study showed that the Amp-FLP in pYNZ22 locus is inherited according to the Mendelian law. The correct genotyping results can be obtained from very little amount of biological material, such as mixed stains, blood stains, single hair and saliva.

关 键 词：pYNZ22位点 扩增片段长度 多态性 银染法 

分 类 号：R89[医药卫生—法医学]