反相HPLC法测定当归芍药冲剂中芍药甙含量  

The Quantitative Determination of Paeoniflorin in Paeonia Lactiflora Pall and Chinese Angelica Granule by HPLC

作　　者：顾小曼[1] 宋利辉[1] 计国琴 李蕾[1] 

机构地区：[1]武汉化工学院,湖北省药材公司科研所

出　　处：《武汉化工学院学报》1995年第1期18-20,共3页Journal of Wuhan Institute of Chemical Technology

摘　　要：用反相高效液相色谱法测定当归芍药冲剂中芍药甙含量。用甲醇—异丙醇—３６％醋酸—水（２５：２：２：７１）作流动相ＵＶ２３０ｎｍ波长检测，ｐｏｌｙｇｏｓｉｌ柱能使芍药甙有很好的分离。在０．４～３．３ｕｇ范围内，峰面积与浓度呈良好的线性关系（ｒ＝０．９９９８）。空白加样回收率为１００．６８％（标准偏差为１．３９％），样品加样回收率为１０１．１６％（标准偏差为１．４４％）。方法简单，精密度较高。A HPLC method was used for the determination of Paeoniflorin in Paeonia Lactilflora Pall and Chinese Angelica granule. Paeoniflorin can be separated on polygosil colymn with a mobile phase of MeOH : (CH3)2CHOH : 36%HAc : H2O=25 : 2 : 2 : 71 and detected at 230nm. The linear relationship between peak area and the concentration (0. 4～3. 3)mg/mL was obtained (r=0. 9998). The recoveries were 100. 68% and 101. 160% respectively.

关 键 词：芍药甙 芍药 当归 高效液相色谱 中成药 

分 类 号：R286.0[医药卫生—中药学]