灵敏的甲状腺微粒体抗体酶联免疫吸附分析的建立  

Detection of Antibodies to Human Thyroid Microsomal Antigen by Sensitive ELISA

作　　者：陆凤先[1] 汤特[1] 王伟[1] 张天庚[1] 叶静[1] 张宝泉[1] 

机构地区：[1]天津医科大学病理生理教研室,天津医科大学总医院核医学科

出　　处：《标记免疫分析与临床》1995年第2期93-95,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：甲状腺微粒体抗体（ＴＭＡｂ）或称甲状腺过氧化物酶抗体（ＴＰＯ－Ａｂ）已广泛用于人类甲状腺自身免疫性疾病的诊断，但粗提的人甲状腺微粒体制备物含有残留的甲状腺球蛋白（ＴＧ）和其它膜抗原，影响方法的灵敏度，我们采用亲和层析的方法，得到纯度较高的ＴＭ抗原，建立了灵敏的ＴＭＡｂ－ＥＬＩＳＡ。该方法的批内变异系数为３．２±０．０１％（ｎ＝８０），批间变异系数为８．３±０．０２％（ｎ＝８）；临床甲亢病人的阳性率为８０．０％（ｎ＝４６），与国内同类放免试剂盒相比，对Ｇｒａｖｅｓ病的阳性检出率提高２０％（ｎ＝２０），桥本氏甲状腺炎的阳性检出率提高１１．７％（ｎ＝２６）。Detection of antibodies to thyroid microsomal antigen (TMAb) or thyroid peroxidase (TPO-Ab) has been widely used in the diagnosis of autoimmune thyroid diseases. Crude human TM contains thyroglobulin (TG) residue and other human membrane antigens so that the sensitivity of TMAb-ELISA may be affected. Highly purified TM was prepared by affinity chromatography and a sensitive TM-ELISA was developed. The intra-assay coefficient of variation was 3. 2±0. 01% (n = 80) and the interassay CV 8. 3±0.02% (n=8). The positivity rate for TMAb in the clinical hyperthyroidism was 80. 4% (n = 46). Compared to other assays developed at home,the positivity rate for TMAb in Graves' disease and Hashimoto's disease was raised by 20% and 11. 7%,respectively.

关 键 词：甲状腺疾病 抗体 微粒体 甲状腺 ELISA 

分 类 号：R581.04[医药卫生—内分泌] R446.6[医药卫生—内科学]