肿瘤坏死因子放射免疫分析的建立及初步临床应用  

Development of RIA for Tumor Necrosis Factor and Its Preliminary Clinical Application

作　　者：黄蓉蓉[1] 陈素娟[1] 杨梅芳[1] 颜光涛[1] 李振甲[1] 

机构地区：[1]中国人民解放军总医院临床基础医学研究所生化研究室

出　　处：《标记免疫分析与临床》1995年第1期33-35,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：用人重组肿瘤坏死因子（ＴＮＦ－α）和特异性兔抗ＴＮＦ血清建立了液相竞争放射免疫分析，最小检出值＞３０ｐｓ／管，标准曲线范围０．３～２４．３ｎｇ／ｍｌ，批内和批间变异系数平均为７．８％和８．４％。本法检测３７名健康献血员血清ＴＮＦ为０．８３±０．２６ｎｇ／ｍｌ，２３例急性黄疸性肝炎、１３例慢性迁延性肝炎、１９例慢性活动性肝炎、２２例肝硬化、２５例肝癌血清ＴＮＦ分别为１．９５±０．５９ｎｇ／ｍｌ，２，０６±０．５１ｎｇ／ｍｌ，１．５８±０．５４ｎｇ／ｍｌ，１．７５±０．６５ｎｇ／ｍｌ和１．５７±０．５３ｎｇ／ｍｌ。２８例肺癌术前血清ＴＮＦ０．９２±０．４２ｎｇ／ｍｌ，２２例肺癌术后血清ＴＮＦ为１．０８±０．２９ｎｇ／ｍｌ。A RIA for tumor necrosis factor (TNF) was developed with rabbit anti TNF antisera.The standard curve covered a range of 0. 3～24. 3ng/ml. The detection limit was<30 pg/tube and the mean intra-and inter-CVs 7. 8% and 8.4%,respectively. Serum level of TNF in 37 healthy subjects was 0. 83±0. 26ng/ml and the highest TNF levels were found in 13 patients with chronic persistent hepatitis (2. 06±0. 51ng/ml),followed by those of 23 patients with acute hepatitis ( 1.95±0. 59ng/ml), 22 with liver cirrhosis (1.75±0. 65ng/ml), 19 with chronic active hepatitis (1.58±0. 54ng/ml) and 25 with hepatoma (1.57±0. 53ng/ml).Serum level of TNF was 0. 92±0. 42ng/ml in 28 patients with lung cancer.

关 键 词：肿瘤坏死因子 放射免疫分析 血清诊断 

分 类 号：R446.112[医药卫生—诊断学] R446.6[医药卫生—临床医学]