大鼠附睾上皮细胞的培养及其功能测定  

Culture of epithelial cells from rat epididymides and estimation of their functional activities

作　　者：张君慧[1] 董赛珍[1] 吴立君[1] 丁之德[1] 谢蔚英 卢琦华[1] 吴明章[1] 

机构地区：[1]上海第二医科大学组织胚胎教研室,上海市计划生育研究所

出　　处：《生殖医学杂志》1995年第4期232-235,共4页Journal of Reproductive Medicine

基　　金：国家计划生育委员会

摘　　要：用酶消化法分离成年雄性ＳＤ大鼠附睾头、体、尾的上皮细胞，贴壁培养。４～１１天后取贴壁细胞进行ＨＥ染色及扫描电镜观察，发现附睾头部和体部大部分为带有微绒毛的主细胞，而尾部细胞较小。并以ＰＮＰＧ为底物测定上清液内α－１．４糖苷酶的活性，结果为体部最高，尾部次之，头部最低。用硫代巴比妥酸法检测上清液中唾液酸的含量，仍以体部最高，但尾部最低。统计学处理均有显著差异。提示大鼠附睾体部可能是精子成熟的关键部位。bjiectives:To study culture of epithelial cells from rat epididymides and their functional activities.Design:Laboratory studies Methods:Cell culture,H. E.staining and scanning electron microscopy of cultured cells,assays of α-1,4 glucosidase and the sialic acid level in culture were performed.Result:Most of the cells from epididymal caput and corpus wereprincipal cells with microvilli on their tree surface.The cells from cauda were relatively small.The level of α-1,4 glucosidase in the corpus was the highest and lowest in the caput.The sialic acid content in the corpus was also the highest,but the lowest in the cauda. Conclusion:Culture of epithelial cells from different segments of rat epididymide was successful.The result of the assays suggested that the rat corpus epididymidis may be the key site for sperm matura-tion.according to the highest α-1, 4 glucosidase and sialic acid levels.

关 键 词：附睾 上皮细胞 葡糖苷酶 唾液酸 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学] R339.21[医药卫生—基础医学]