cAMP介导内皮素－1对大鼠黄体细胞孕酮分泌的作用  

The mediation of cAMP in the endothelin-l action on progesterone secretion by rat luteal cells

作　　者：徐斯凡[1] 郑月慧[1] 赵白鸽 王忠兴 周寿康 赵伟 吴晓芸 

机构地区：[1]江西医学院生理教研室,上海市计划生育科学研究所

出　　处：《生殖医学杂志》1995年第2期88-91,共4页Journal of Reproductive Medicine

基　　金：国家自然科学基金及江西省自然科学基金

摘　　要：本研究采用大鼠黄体细胞体外孵育技术，观察了缩血管肽内皮素－１（ＥＴ－１）对黄体细胞孕酮分泌的影响及其与细胞内信使ｃＡＭＰ的相互关系。结果表明，１０－６ｍｏｌ／Ｌ的ＥＴ－ｌ可显著抑制黄体细胞的基础孕酮分泌和ｈＣＧ诱导的孕酮分泌；外源性ｃＡＭＰ和腺苷酸环化酶激动剂Ｆｏｒｓｋｏｌｉｎ则可促进黄体细胞分泌孕酮；当ｃＡＭＰ或Ｆｏｎｓｋｏｌｉｎ与ＥＴ－１共同作用时，前者可完全反转ＥＴ－１对黄体细胞的抑制作用，孕酮分泌恢复到对照组水平。提示Ｅｌ－１可能是一种卵巢局部调节因子，通过抑制黄体细胞内的腺苷酸环化酶，减少ｃＡＭＰ的生成和释放，进而抑制黄体细胞的孕酮分泌。Objective :To determine the effect of endothelin-l on the progesterone( P)ecretion by rat luteal cells and its relation with cAMP. Design :In vitro experimental study. Setting:Laboratory in Shanghai lnstitute of Planned Parenthood Research. Methods:Preparation of luteal cells suspension from Sprague-Dawley rat ovaries and incubation in KR-BGA1 solution,Samples of incubation medium were collected and stored at-20℃for measurement of Pand cAMP with radioimmunoassay. Results:Endothelin-l at a concentration of l0-6mol/L significantly reduced the basal and hCG inducedP secretion about 20%and29%respectively (P<0. 0l ).xogenous cAMP significantly increased the p se-cretion to about 22%of the control group(P<0.05).When added to the endothelin-1 treated medium theP secretion returned to the control level. Forskolin stimulated the P secretion to 2.l times higher than thecontrol (P<0. 0l ),and when added to the endothelin-l culture system,the P secretion increased to a levelhigher than the control (P<0.01).The cAMP concentration in the luteal cell mediuM was significantly re-duced(P<0.05)when culltured with endothelin-l. Conelusion :The study suggested that endothelin-1 may be an intra- ovarian regulatory factor which in-hibits progesterone secretion from rat luteal cells by suppressing the adenyl cyclase activity and reducingcAMP accumulation .

关 键 词：内皮素 CAMP 孕酮 黄体 

分 类 号：R335.5[医药卫生—人体生理学]