胰岛素替代治疗对青春期糖尿病鼠睾丸间质和支持细胞功能的影响  

Effect of insulin replacement on Leydig and Sertoli cell function in pubertal rats with diabetes

作　　者：张家颖[1] 王忠山[1] 王苹[1] 许宗革[1] 

机构地区：[1]长春白求恩医科大学分子生物学中心

出　　处：《生殖医学杂志》1995年第4期229-231,共3页Journal of Reproductive Medicine

基　　金：国家自然科学基金

摘　　要：以４０日龄雄性Ｗｉｓｔａｒ大鼠４０只，分为对照（Ｃ），糖尿病（Ｄ），糖尿病胰岛素及时治疗（ＤＣＩＲ）和糖尿病胰岛素延迟治疗（ＤＤＩＲ）组，进行睾丸间质细胞和支持细胞功能的研究。结果表明：血清睾酮Ｄ组最低，培养间质细胞睾酮分泌量ＤＣＩＲ、ＤＤＩＲ和Ｄ组均显著低于Ｃ组（Ｐ＜０．０５及０．００１），ｃＡＭＰ分泌量ＤＤＩＲ和Ｄ组显著低于Ｃ和Ｄ分别的ＣＩＲ组（Ｐ＜０．０１）；睾丸组织雄激素结合蛋白（ＡＢＰ）水平，ＤＤＩＲ组显著高于Ｃ和Ｄ组（分别为Ｐ＜０．０５及Ｐ＜０．０１），其它组间比较差异不显著，培养支持细胞ＡＢＰ分泌量Ｄ组显著低于Ｃ和ＤＣＩＲ组（Ｐ＜０．０５），ｃＡＭＰ分泌量ＤＤＩＲ和Ｄ组显著低于Ｃ和ＤＣＩＲ组（Ｐ＜０．０１）。bjectives:To study the effect of diabetes on function of Leydig and Sertoli cells in pubertal rats and the action of insulin replacement.Design:Experimental study of cell biology.Setting:Laboratory study of Cell Biology in Medical University.Methods:The animals were divided into control(C), diabetes withcontinuous insulin replacement(DCIR),diabetes with delayed insulin replacement(DDIR)and diabetes(D)groups. Testosterone(T)serum and Leydig cell cultured media,androgen binding protein(ABP)in teatis and Sertoli cell cultured media and cAMP in Leydig and Sertoli cell cultured media were measured.Results:Serum T in group D was significantly lower than those in the other three groups.In culture media T secretion on groups DCIR, DDIR and D were significantly lower than that in group C,the cAMP secretion in groups D and DDIR were also lower than those in groups C and DCIR in Leydig cell culture me-dia.The testis ABP in group DDIR was higher than those in other groups. While in culture media ABP se-cretion in group D was significantly lower than those in C and DCIR. The cAMP levels in groups D and DDIR were also lower than those in other groups.Conclusion:Diabetes in pubertal rats impaired Leydig and Sertoli cell function. Insulin replacement im-proved the deficits,but didn’t reach the normal levels. The effect of continuous insulin replacement was better than delayed insulin replacement.

关 键 词：青春期 糖尿病 间质细胞 支持细胞 睾丸 

分 类 号：R587.105[医药卫生—内分泌] R697.220.5[医药卫生—内科学]