地高辛标记探针原位杂交检测肝组织中丙型肝炎病毒RNA  

DETECTION OF HEPATITIS C VIRUS RNA IN LIVER WITH DIGOXININ LABELLED PROBE

作　　者：张长法[1] 周永兴[1] 王春杰[1] 姚志强[1] 

机构地区：[1]中国人民解放军南京空军医院,第四军医大学唐都医院,第四军医大学病理教研室

出　　处：《肝脏病杂志》1995年第1期10-12,共3页

摘　　要：用地高辛标记ＨＣＶ５’—ＮＣ区ｃＤＮＡ探针原位杂交检测了２４例ＨＣＶ感染的慢性肝病患者肝组织中ＨＣＶＲＮＡ。结果：１７例同时血清抗ＨＣＶ（ⅡＥＬＩＳＡ法）和ＨＣＶＲＮＡ（套式ＰＣＲ法）均阳性病人中，１４例（８２．３％）肝组织检出了ＨＣＶＲＮＡ。７例仅有抗ＨＣＶ阳性病人，肝组织中没有检出ＨＣＶＲＮＡ。ＨＣＶＲＮＡ特异性信号主要位于肝细胞浆。感染ＨＣＶ的肝细胞呈散在、灶状或弥漫形式分布，与ＡＬＴ水平、病理改变没有明显联系。感染ＨＣＶ肝细胞周围见较多的淋巴细胞和单核细胞浸润。结果提示ＨＣＶ在肝细胞浆复制，诱导宿主免疫反应，在慢性ＨＣＶ感染的发病机理中，起着重要的作用。Nonisotopic in situ hybridization using a digoxinin labelled cDNA probe of5’-NC region of HCV wasperformed on liver tissue from 24 HCV infection patients with chronic liver disease。Result :in l4(82.3%)of l7 patients with HCV infection(Both Ⅱ ELISA Anti-HCV and nested PCR HCV RNA postivein serum)HCV RNA were detected in liver tissue,but undetectable in 7 patients with anti-HCV postiveonly。 HCV RNA were mainly localized in the cytoplasm of hepatocytes,The distributions of the HCVinfected hepatocytes were revealed of spotty, scattered or diffuse patterns。 There was no correlation be-tween these distributions and the levels of ALT pathologic changes of liver。 There were many lym-phocytes and mononuclear cells infiltration in periphery of HCV-infected liver cells。 The results suggest-ed that the replication of HCV in the cytoplasm of hepatocytes elicited the host immune response to cellsinfected with HCV and play an important role in pathogenesis of HCV infection.

关 键 词：丙型肝炎病毒 核酸杂交 地高辛配基 病理学 

分 类 号：R512.630.2[医药卫生—内科学]