L─谷氨酰胺生物传感器的研制和应用  

TISSUE MEMBRANE ELECTRODE SENSITIZED FOR L-GLUTAMINE

作　　者：马万红[1] 仰蜀薰[1] 孙炳耀[1] 石杰[1] 邱宏斌 

机构地区：[1]郑州大学化学系

出　　处：《郑州大学学报（自然科学版）》1995年第4期72-76,共5页Journal of Zhengzhou University (Natural Science)

摘　　要：利用猪肾、猪肝组织为酶源偶合氨气敏电极研制了Ｌ一谷氨酰胺生物传感器。研究和讨论了该组织传感器的最佳实验条件，比较了猪肾组织与猪肝组织传感器的响应特性以及组织不同部位切片制成传感器的响应差别．对Ｌ一谷氨酰胺响应范围为１．８×１０￣（－５）－２．５×１０￣（－２）ｍｏｌ／Ｌ，检测下限为１．１×１０￣（－５）ｍｏｌ／Ｌ，响应斜率为５０．０ｍｖ／ｄｅｃａｄｅ（３０℃），该传器已用于板兰根样品测定。A new biosenser based on porky kidney and liver tissue for L-glutamine,cou-pling with potentiometric ammonia gas electrode,has been designed and constructed.Thedifference between kidney and liver tissue electrode for sensing glutamine was investigat-ed.The biosenser has linear respones in the range of the substrate concentratiOn of 1.8×10￣(-5)～2.5×10￣(-2)mol/L. The detection limit is 1.1×10￣(-5)mol/L and the response slope50. 0mV/p Glu.It has a useful lifetime at least 42 days. This methode is compared withthat of ion exchange chromatography for the determination of L-glutamine with satisfacto-rv result.

关 键 词：生物传感器 组织电极 L-谷氨酰胺 测定 

分 类 号：Q－331[生物学] O623.626[理学—有机化学]