低温对原代星形胶质细胞的胶质纤维酸性蛋白和微丝的影响  

EFFECTS OF HYPOTHERMIA ON GLIAL FIBRILLARY ACIDIC PROTEIN AND MICRORILAMENTS IN PRIMARY CULTURED ASTROCYTES

作　　者：吉宏龙 李天麟[1] 王天佑[1] 李玉瑞[1] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所,北京神经外科研究所

出　　处：《解剖学报》1995年第1期67-70,T012,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金

摘　　要：用荧光染色方法，观察了低温（２～２２℃）对原代培养的星形胶质细胞的胶质纤维酸性蛋白（ＧＦＡＰ）和微丝（ＭＦ）的影响。发现在低于２２℃中处理后，ＧＦＡＰ束和ＭＦ束变细，ＭＦ数量减少。处理时间越长，变化越明显。形态分化良好的细胞的ＧＦＡＰ束耐低温能力提高。结果表明，低温时ＧＦＡＰ束间联结断裂，而其本身无解聚；ＭＦ束间联结断裂，ＭＦ本身出现解聚。By using of immunocytochemical method,glial fibrillary acidic protein(GFAP)and microfilaments(MF)in primary cultured astrocytes from cerebrum of neonatal rats treated at different low temperature(2 ,12 , 22℃)were studied.It was shown that upon exposure to hypothermia below 22℃,Both GFAP bundles and microfilament bundles became thinner,and the amount of MF decreased.These changes were more remarkable when the cold treatment was longer.GFAP of well morphologically differentiated astrocytes showed higher tolerance to hypothermia.The results suggest that hypothermia may lead to break-down of'ross-bridges'among GFAP bundles but not depolymerization of GFAP itself,whereas'cross-bridges'among MF bundles break down and MF deploymerize progressively.

关 键 词：低温 微丝 神经胶质 蛋白质 星形细胞 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学] R318.52[医药卫生—基础医学]