三种甲替溶解剂在MTT比色法中的应用  

APPLICATION OF THREE SOLUBILIERS OF FORMAZAN IN MTT COLORIMETRIC ASSAY

作　　者：郝京生[1] 贾东华[1] 董玉环[1] 

机构地区：[1]北京军区军事医学研究所

出　　处：《河北医学院学报》1995年第6期324-326,共3页

摘　　要：研究ＭＴＴ比色法中三种甲　溶解剂，异丙醇（ＩＳＯＰ）、１０％曲拉通—５０％异丙醇（ＴＲＩ－ＴＯＮ－ＩＳＯＰ）和２０％ＳＤＳ（均加入１０ｍｍｏｌ／ＬＨＣｌ酸化）的应用效果。结果发现，在测定不同浓度的外周血单个核细胞（ＰＢＭＣ）时，ＴＲＩＴＯＮ—ＩＳＯＰ与ＳＤＳ效果相当，而在测定不同浓度的Ｈｅｌａ细胞时，前者优于后者，而ＴＲＩＴＯＮ—ＩＳＯＰ和ＳＤＳ均优于ＩＳＯＰ。The effects of three solubilizers of formazan which were iospropanol (ISOP), 10% triton-50% iospropanol (TRITON-ISOP) and 20% sodium dodecyl sulphate (SDS) (all with 10mmol/L HCL) in MTT assay were studied. The results showed the effects of TRITON-ISOP and SDS were similar in determining the concentration of PBMC. TRITON-ISOP was better than SDS, TRITON-ISOP and SDS were superior to ISOP in determining the concentration of Hela cells.

关 键 词：MTT比色法 甲Za溶解剂 异丙醇 曲拉通 SDS 

分 类 号：R446.63[医药卫生—诊断学] R730.43[医药卫生—临床医学]