用猪卵透明带55KDa糖蛋白抗原（ZP3）免疫恒河猴的初步研究  

A Prdliminary Immunization Study on Rhesus Monkeys Using The 55KDa Porcine Zona Pellucida Antigen(ZP3)

作　　者：潘善培[1] 谢琪璇[1] 陈海玲[1] 刘学高[1] 陈乾生[1] 马昭林 梁兴林 

机构地区：[1]暨南大学生殖免疫研究中心,广东省华南濒危动物研究所

出　　处：《暨南大学学报（自然科学与医学版）》1995年第1期90-97,共8页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金资助项目

摘　　要：用猪卵透明带５５ＫＤａ糖蛋白抗原（ＺＰ３）和胞壁酰肢二肽佐剂（ＭＤＰ）免疫３只雌性恒河猴，研究猪ＺＰ３免疫的抗生育作用及其对卵巢正常结构与功能的影响，评价用恒河猴作卵透明带生育调节疫苗研究动物模型的合适性。实验观察持续４４０天，三只免疫猴中，两只产生高滴度抗ＺＰ３抗体并导致不孕（免疫组怀孕率为３３％，对照组为８０％）．血清Ｅ＿２和Ｐ测定结果表明，抗体滴度高的两只猴Ｅ＿２和Ｐ的水平极低，且不呈周期性变化，组织学观察表明两只不孕猴的卵巢缺乏生长卵泡和成熟卵泡，揭示ＺＰ３免疫阻断了卵泡的发育，这些结果与用猪ＺＰ３免疫其它灵长类动物报导的相似，它进一步证实ＺＰ３免疫的抗生育作用，并支持用恒河猴作动物模型，研究用合成肽段或基因重组产物作免疫原的卵透明带生育调节疫苗。Components of the porcine zona pellucida are under investigation as potential target antigensfor contraceptive vaccine development.To evaluate the suitalility of the rhesus monkey as ananimal model for future immnocontraceptive studies utilzing synthetic or recombinant zonacomponents,thase females.were immunized with porcine ZP3(Mr=55 000)and their immuneiesponses, endocrine(estradiol,progesterone)profiles,ovarian histology and fertility weremonitored over a 440 day period.Two of the thase immunized females developed highantibody titers and became infertile(33% fertility rate versus 80% in non一immunized controlgroup).While steroid profiles.in all immunzied monkeps were abnormal,the profiles,of the twomonkeys with the high titers differed significantly from the monkey with the lower antibodytiters. Histological examination of ovaries,from two infertile monkeys indicated an abence ofmature and growing follicles suggesting a disruption in folliculogenesis.Thase.data are similarto those reported for other primates,immnunized with porcine zona and support the utilizationof the Rhesus monkey in zona-based immunocontraceptive investigations.

关 键 词：卵透明带 生殖免疫 恒河猴 糖蛋白抗原 免疫 

分 类 号：Q959.848[生物学—动物学]