乳突类圆线虫病人工免疫研究初报  

IMMUNOLOGICAL STUDIES ON STRONGYLOIDES PAPILLOSUS INFECTION

作　　者：蒋金书[1] 陈刚[1] 韩谦[1] 

机构地区：[1]北京农业大学兽医学院

出　　处：《畜牧兽医学报》1989年第2期162-167,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：用Co^(60)4000伦琴(R)、20000伦琴和40000伦琴照射乳突类圆线虫的丝状幼虫以及用丝状幼虫的冻融提取物经一次和二次免疫接种家兔,对20×10~4条丝状幼虫的攻毒均显示有一定的保护力。二次免疫优于一次免疫,4000R、40000R的二次免疫与一次免疫的荷虫量相比差异显著(P<0.05);4000R一次、二次免疫,20000R二次免疫和40000R一次、二次免疫与对照组相比,其荷虫量的差异极显著(P<0.01);40000R二次免疫的荷虫量明显地少于20000R和4000R二次免疫的荷虫量。虫卵检查的结果亦与此相符。抗原提取物一次、二次免疫的荷虫量与对照组相比差异极显著(P<0.01),但在剖检时仍发现有多量虫体,这可能是由于免疫的抗原量不足所造成的。 经检查确定对照组的排卵时间为9天整,4000R组平均为9.67天,20000R组平均为10.67天,40000R组平均为12天。 一条寄生虫雌虫一昼夜可排卵13～24个,平均为18个。Immunization with 2000 filariform larvae Strongyloides papillosus irradiated with Co80 at 4 Krad, 20 Krad or 40 Krad respectively, and immunization with freeze-thaw extraction of filariform larvae produced considerable levels of resistance to the challenge of 20 × 104 filariform larvae. Our experiment showed that the results of secondary immunization was better than that of the primary. The adult worm burdence of secondary immunization with irradiated larvae at 4 Krad and 40 Krad was significantly less than that of the primary ( P<0.05 ) when compared all the vaccinated groups with the controls the difference was very significant ( P<0.01 ) . Among all groups the adult worm burdence in secondary immunization groups with 2000 filariform larvae irradiated at 40 Krad was the least one. The results of fecal egg counts (e.p.g. ) were consistent with those of adult worm burdence.The adult worm burdence in groups immunized with the extraction of filariform larvae was less than that of the controls ( P<0.01 ) . The first eggs of control group appeared in the feces on day 9 after infection,the 4000R group on day 9.67,the 20000R group on day 10.67 and the 40000R group on day 12.

关 键 词：类圆线虫病 人工免疫 

分 类 号：S855.910.3[农业科学—临床兽医学]